Department of Photonics, Center for Micro/Nano Science and Technology, and Advanced Optoelectronic Technology Center, National Cheng Kung University , 701 Tainan, Taiwan.
Institute of Biomedical Engineering & Molecular Imaging Center, National Taiwan University , Taipei 106, Taiwan.
ACS Appl Mater Interfaces. 2015 Nov 18;7(45):25259-69. doi: 10.1021/acsami.5b07110. Epub 2015 Nov 9.
We have successfully introduced a proton-induced controlled reaction of HAuCl4 and poly(styrene-alt-maleic acid) (PSMA) sodium salt to prepare triangular and multicore Au@polymer nanoparticles (NPs). The interparticle interactions in the core gave rise to an absorption band at the near-infrared wavelength. The near-infrared optical properties of the resulting Au-polymer nanostructures are highly stable in a physiological environment, which offered strong photo-to-thermal conversion by a moderate continuous-wave 808 nm laser and exhibited multiphoton fluorescence for imaging using a 1230 nm light excitation (femtosecond laser). Exposure of the carboxylate groups at the polymer shell made the surface structure of the Au multicore @polymer NPs directly conjugate Pt(II)-/Pt(IV)-based drugs, which possessed the elimination of the immediate toxicity over the short time and resulted in an anticancer effect after 3 days. A synergistic effect of the chemo-photothermal therapy showed a moderate hyperthermia assistance (<1 W/cm(2)) and better anticancer performance over time compared with the individual treatments. We demonstrated that such PSMA-based methodology not only enables a broad range of chemical material synthesis in the kinetic control to form Au nano-octahedrons and nanotriangles using Br(-)/I(-) ions additives but also could be extended to form Au/Fe3O4@polymer nanocomposites via proton-assisted PSMA self-assembly.
我们成功地引入了质子诱导的 HAuCl4 和聚（苯乙烯-交替-马来酸）（PSMA）钠盐的可控反应，制备了三角形和多核 Au@聚合物纳米粒子（NPs）。核内粒子间的相互作用导致了近红外波长的吸收带。所得 Au-聚合物纳米结构在生理环境中具有高度稳定的近红外光学性质，通过适度的连续波 808nm 激光实现了强光热转换，并使用 1230nm 光激发（飞秒激光）显示了多光子荧光成像。聚合物壳上的羧酸盐基团的暴露使 Au 多核@聚合物 NPs 的表面结构能够直接与 Pt(II)/Pt(IV)基药物缀合，这在短时间内消除了即时毒性，并在 3 天后产生了抗癌效果。化学光热治疗的协同作用显示出适度的热疗辅助（<1 W/cm(2))，并且随着时间的推移，抗癌性能优于单独治疗。我们证明，这种基于 PSMA 的方法不仅能够在动力学控制下广泛合成化学物质，形成 Au 纳米八面体和纳米三角形，使用 Br(-)/I(-)离子添加剂，还可以通过质子辅助 PSMA 自组装扩展到形成 Au/Fe3O4@聚合物纳米复合材料。
