Tropical Disease Research Center and Microbiology Faculty, University of Costa Rica, San José, Costa Rica.
J Food Prot. 2013 May;76(5):879-82. doi: 10.4315/0362-028X.JFP-12-400.
Arcobacter is a genus of growing importance worldwide; some of its species are considered emerging enteropathogens and potential zoonotic agents. In Costa Rica, as well as in other countries, its isolation has been reported, so the objective of this project was to evaluate and identify the presence of Arcobacter in chicken viscera sold in the metropolitan area of San José, Costa Rica, as well as to determine the antimicrobial resistance patterns associated with it. One hundred fifty samples of chicken viscera including heart, liver, and other gastrointestinal organs were purchased from 15 supermarkets and 15 local retailers. De Boer and Houf broths were used as enrichment media; isolation was done with Arcobacter-selective medium and with membrane filtration with blood agar. Typical colonies were identified with genus-specific PCR, and species identification was made with multiplex PCR. Susceptibility to ampicillin, ciprofloxacin, chloramphenicol, erythromycin, gentamicin, and tetracycline was done with the Epsilometer test. The isolation frequency of Arcobacter genus obtained in this study was of 17.3%. A total of 33 isolates were obtained from the poultry samples, and according to the multiplex PCR methodology, 22 (66.7%) isolates were identified as Arcobacter butzleri, 8 (24.2%) as Arcobacter cryaerophilus, and 1 (3.1%) as Arcobacter skirrowii. Two strains were not identified. No statistical significant difference was found when the source of samples was compared. Resistance toward chloramphenicol was 68.75%, followed by ampicillin (43.75%) and ciprofloxacin (18.75%); all strains were susceptible to tetracycline.
弯曲杆菌属是一个在全球范围内日益重要的属；其一些物种被认为是新兴的肠道病原体和潜在的人畜共患病原体。在哥斯达黎加以及其他国家，已经报告了该属的分离情况，因此本项目的目的是评估和确定哥斯达黎加圣何塞大都市区销售的鸡内脏中是否存在弯曲杆菌属，以及确定与之相关的抗生素耐药模式。从 15 家超市和 15 家当地零售商购买了包括心脏、肝脏和其他胃肠道器官在内的 150 份鸡内脏样本。使用 De Boer 和 Houf 肉汤作为富集培养基；使用弯曲杆菌选择性培养基和血琼脂膜过滤进行分离。使用属特异性 PCR 鉴定典型菌落，并用多重 PCR 进行种鉴定。使用 Epsilometer 试验测定对氨苄西林、环丙沙星、氯霉素、红霉素、庆大霉素和四环素的敏感性。本研究中获得的弯曲杆菌属的分离频率为 17.3%。从禽类样本中总共获得了 33 个分离株，根据多重 PCR 方法，22 个（66.7%）分离株鉴定为 Butzleri 弯曲杆菌，8 个（24.2%）鉴定为 Cryaerophilus 弯曲杆菌，1 个（3.1%）鉴定为 Skirrowii 弯曲杆菌。有 2 个菌株未鉴定。当比较样本来源时，未发现统计学上的显著差异。对氯霉素的耐药率为 68.75%，其次是氨苄西林（43.75%）和环丙沙星（18.75%）；所有菌株均对四环素敏感。
