Kabeya Hidenori, Maruyama Soichi, Morita Yukio, Ohsuga Tomoko, Ozawa Saori, Kobayashi Yoko, Abe Maiko, Katsube Yasuji, Mikami Takeshi
Laboratory of Veterinary Public Health, Department of Veterinary Medicine, College of Bioresource Sciences, Nihon University, 1866 Kameino, Fujisawa, Kanagawa 252-8510, Japan.
Int J Food Microbiol. 2004 Feb 1;90(3):303-8. doi: 10.1016/s0168-1605(03)00322-2.
A survey was conducted to examine the prevalence of Arcobacter species among meat samples and to investigate the antimicrobial susceptibility of the isolates in Japan. In 1998 and 1999, samples of beef (n=90), pork (n=100) and chicken meat (n=100) were purchased from seven retail shops. Arcobacter species were isolated from 2.2%, 7.0% and 23.0% of beef, pork and chicken meat samples, respectively. The rate of isolations in chicken meats was shown to be significantly higher than those of beef and pork. Species-specific polymerase chain reaction (PCR) demonstrated that the most dominant Arcobacter species was Arcobacter butzleri among the isolates examined. Multiple contaminations with different Arcobacter species were observed in 5% of the chicken samples. Almost all the strains tested showed resistance to vancomycin (100%) and methicillin (97.5%). Strains resistant to cephalothin, sulfamethoxazole-trimethoprim, nalidixic acid and chloramphenicol were detected at the rate of 81.1%, 67.2%, 53.5% and 24.6%, respectively. All Arcobacter strains examined were susceptible to ampicillin, tetracycline, streptomycin and kanamycin.
开展了一项调查，以检测日本肉类样本中弓形杆菌属的流行情况，并研究分离菌株的抗菌药敏性。1998年和1999年，从七家零售店购买了牛肉样本（n = 90）、猪肉样本（n = 100）和鸡肉样本（n = 100）。弓形杆菌属分别从2.2%的牛肉样本、7.0%的猪肉样本和23.0%的鸡肉样本中分离得到。鸡肉样本中的分离率显著高于牛肉和猪肉样本。种特异性聚合酶链反应（PCR）表明，在所检测的分离菌株中，最主要的弓形杆菌属是布氏弓形杆菌。在5%的鸡肉样本中观察到不同弓形杆菌属的多重污染。几乎所有测试菌株都对万古霉素（100%）和甲氧西林（97.5%）耐药。检测到对头孢噻吩、磺胺甲恶唑-甲氧苄啶、萘啶酸和氯霉素耐药的菌株，其比例分别为81.1%、67.2%、53.5%和24.6%。所有检测的弓形杆菌菌株对氨苄西林、四环素、链霉素和卡那霉素敏感。
