Sturrock Anna M, Hunter Ewan, Milton J Andy, Trueman Clive N
National Oceanography Centre Southampton, Waterfront Campus, University of Southampton, Southampton SO14 3ZH, UK; Centre for Environment, Fisheries and Aquaculture Science, Lowestoft Laboratory, Lowestoft, Suffolk NR33 0HT, UK.
J Trace Elem Med Biol. 2013 Oct;27(4):273-85. doi: 10.1016/j.jtemb.2013.03.001. Epub 2013 Mar 14.
A comprehensive review of the analytical literature revealed substantial under-representation of trace element concentrations in fish blood, particularly for marine species. We describe a simple dilution procedure to measure Li, Mg, K, Ca, Mn, Cu, Zn, Se, Rb, Sr, Ba and Pb concentrations in low volumes of blood plasma of adult plaice (Pleuronectes platessa) using high resolution-inductively coupled plasma-mass spectrometry (HR-ICP-MS). Captive male and female plaice (n = 18) were serially sampled for one year and samples collected outside of the spawning season (n = 157) used to estimate reference ranges for this species. Method accuracy was deemed satisfactory, based on its application to the analysis of a certified reference material. Precision was generally <3%, with the most conservative measure of precision being ≤10% for all elements except Pb (∼20%). This is the first study to analyse fish blood plasma by ICP-MS and includes some of the first reference ranges for trace element concentrations in fish blood.
对分析文献的全面回顾显示，鱼类血液中微量元素浓度的研究严重不足，尤其是海洋物种。我们描述了一种简单的稀释程序，使用高分辨率电感耦合等离子体质谱法（HR-ICP-MS）测量成年鲽鱼（Pleuronectes platessa）少量血浆中锂、镁、钾、钙、锰、铜、锌、硒、铷、锶、钡和铅的浓度。对圈养的雄性和雌性鲽鱼（n = 18）进行了为期一年的连续采样，并使用产卵季节以外收集的样本（n = 157）来估计该物种的参考范围。基于该方法在分析一种有证标准物质中的应用，认为方法准确性令人满意。精密度一般<3%，除铅（约20%）外，所有元素最保守的精密度测量值≤10%。这是第一项通过ICP-MS分析鱼类血浆的研究，并且包括了一些鱼类血液中微量元素浓度的首批参考范围。
