Research Institute for Fragrance Materials, Inc., Woodcliff Lake, NJ 07677, USA.
Food Chem Toxicol. 2013 Sep;59:784-92. doi: 10.1016/j.fct.2013.04.040. Epub 2013 Apr 30.
Genotoxicity studies were conducted on a group of 8 fragrance ingredients that belong to the nitrile family. These nitriles are widely used in consumer products however there is very limited data in the literature regarding the genotoxicity of these nitriles. The 8 nitriles were assessed for genotoxicity using an Ames test, in vitro chromosome aberration test or in vitro micronucleus test. The positive results observed in the in vitro tests were further investigated using an in vivo micronucleus test. The results from these different tests were compared and these 8 nitriles are not considered to be genotoxic. Dodecanitrile and 2,2,3-trimethylcyclopent-3-enylacetonitrile were negative in the in vitro chromosome aberration test and in vitro micronucleus test, respectively. While citronellyl nitrile, 3-methyl-5-phenylpentanenitrile, cinnamyl nitrile, and 3-methyl-5-phenylpent-2-enenitrile revealed positive results in the in vitro tests, but confirmatory in vivo tests determined these nitriles to be negative in the in vivo micronucleus assay. The remaining two nitriles (benzonitrile and α-cyclohexylidene benzeneacetonitrile) were negative in the in vivo micronucleus test. This study aims to evaluate the genotoxicity potential of these nitriles as well as enrich the literature with genotoxicity data on fragrance ingredients.
对一组属于腈家族的 8 种香料成分进行了遗传毒性研究。这些腈广泛用于消费产品，但关于这些腈的遗传毒性的文献数据非常有限。使用 Ames 试验、体外染色体畸变试验或体外微核试验评估了这 8 种腈的遗传毒性。在体外试验中观察到的阳性结果进一步通过体内微核试验进行了研究。比较了这些不同试验的结果，这 8 种腈不被认为具有遗传毒性。十二腈和 2,2,3-三甲基环戊-3-烯基乙腈在体外染色体畸变试验和体外微核试验中均为阴性。虽然柠檬腈、3-甲基-5-苯基戊腈、肉桂腈和 3-甲基-5-苯基戊-2-烯腈在体外试验中呈现阳性结果，但确认性体内试验确定这些腈在体内微核试验中为阴性。其余两种腈（苯腈和α-环己基苯乙腈）在体内微核试验中为阴性。本研究旨在评估这些腈的遗传毒性潜力，并为香料成分的遗传毒性数据文献增砖添瓦。
