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核心技术专利：CN118964589B侵权必究

核心技术专利：CN118964589B侵权必究

Castro R, Brito B, Notario V

Department of Biochemistry, Georgetown University Medical School, Washington, D.C. 20007.

Cell Mol Neurobiol. 1990 Jun;10(2):281-9. doi: 10.1007/BF00734581.

Previous studies have reported a marked reduction in the [3H]thymidine incorporation in forebrain after administration of a dopamine antagonist such as haloperidol. 2. We have investigated the possibility that the expression levels of genes related to DNA metabolism could be altered by haloperidol treatment. 3. By Northern blot analysis, we have studied the steady-state mRNA levels for genes involved in DNA metabolism, in neonate rat mesencephalon and forebrain, after chronic prenatal blockade of dopamine receptors with haloperidol. 4. We found that the expression levels for DNA polymerases alpha and beta were clearly reduced in forebrain by haloperidol treatment. On the contrary, the expression of DNA polymerase beta was increased in mesencephalon. 5. Our results suggest that dopamine receptors occupancy may be a critical factor in controlling cell proliferation during brain development, through a mechanism(s) involving changes in the expression of DNA polymerases.

先前的研究报道，给予多巴胺拮抗剂（如氟哌啶醇）后，前脑[3H]胸腺嘧啶核苷掺入量显著降低。2. 我们研究了氟哌啶醇治疗可能改变与DNA代谢相关基因表达水平的可能性。3. 通过Northern印迹分析，我们研究了在新生大鼠中脑和前脑，用氟哌啶醇对多巴胺受体进行慢性产前阻断后，参与DNA代谢的基因的稳态mRNA水平。4. 我们发现，氟哌啶醇治疗使前脑中DNA聚合酶α和β的表达水平明显降低。相反，中脑中DNA聚合酶β的表达增加。5. 我们的结果表明，多巴胺受体占据可能是通过涉及DNA聚合酶表达变化的一种或多种机制，在脑发育过程中控制细胞增殖的关键因素。

Castro R, Brito B, Notario V

Department of Biochemistry, Georgetown University Medical School, Washington, D.C. 20007.

Cell Mol Neurobiol. 1990 Jun;10(2):281-9. doi: 10.1007/BF00734581.

Previous studies have reported a marked reduction in the [3H]thymidine incorporation in forebrain after administration of a dopamine antagonist such as haloperidol. 2. We have investigated the possibility that the expression levels of genes related to DNA metabolism could be altered by haloperidol treatment. 3. By Northern blot analysis, we have studied the steady-state mRNA levels for genes involved in DNA metabolism, in neonate rat mesencephalon and forebrain, after chronic prenatal blockade of dopamine receptors with haloperidol. 4. We found that the expression levels for DNA polymerases alpha and beta were clearly reduced in forebrain by haloperidol treatment. On the contrary, the expression of DNA polymerase beta was increased in mesencephalon. 5. Our results suggest that dopamine receptors occupancy may be a critical factor in controlling cell proliferation during brain development, through a mechanism(s) involving changes in the expression of DNA polymerases.

先前的研究报道，给予多巴胺拮抗剂（如氟哌啶醇）后，前脑[3H]胸腺嘧啶核苷掺入量显著降低。2. 我们研究了氟哌啶醇治疗可能改变与DNA代谢相关基因表达水平的可能性。3. 通过Northern印迹分析，我们研究了在新生大鼠中脑和前脑，用氟哌啶醇对多巴胺受体进行慢性产前阻断后，参与DNA代谢的基因的稳态mRNA水平。4. 我们发现，氟哌啶醇治疗使前脑中DNA聚合酶α和β的表达水平明显降低。相反，中脑中DNA聚合酶β的表达增加。5. 我们的结果表明，多巴胺受体占据可能是通过涉及DNA聚合酶表达变化的一种或多种机制，在脑发育过程中控制细胞增殖的关键因素。