Alberch J, Brito B, Notario V, Castro R
Departament de Biologia Cel.lular i Anatomia Patològica, Hospital de Bellvitge, Facultat de Medicina, Universitat de Barcelona, Spain.
Neurosci Lett. 1991 Oct 14;131(2):228-32. doi: 10.1016/0304-3940(91)90620-9.
Pregnant rats were treated daily with haloperidol (2 mg/kg) for 11 days until 1 day before birth. The levels of nerve growth factor (NGF), its receptor (NGFR) and NGFR-mRNA were measured in forebrain of 2-day old postnatal rats. Using Northern blot analysis, we observed a decrease in NGFR-mRNA. Furthermore, in binding studies, Kd and Bmax of treated rats were lower than in controls, but only in the low affinity binding sites. However NGF and its mRNA did not change after haloperidol treatment. In conclusion, our results suggest that prenatal haloperidol treatment can modify the development of forebrain cells, by changing NGFR expression.
怀孕大鼠每天用氟哌啶醇（2毫克/千克）治疗11天，直至出生前1天。在出生后2天的大鼠前脑中测量神经生长因子（NGF）、其受体（NGFR）和NGFR-mRNA的水平。通过Northern印迹分析，我们观察到NGFR-mRNA减少。此外，在结合研究中，治疗组大鼠的解离常数（Kd）和最大结合容量（Bmax）低于对照组，但仅在低亲和力结合位点。然而，氟哌啶醇治疗后NGF及其mRNA没有变化。总之，我们的结果表明，产前氟哌啶醇治疗可通过改变NGFR表达来改变前脑细胞的发育。
