Department of Otorhinolaryngology Head and Neck Surgery, Ordu University Medical School, Teaching and Educational Hospital, 52200, Ordu, Turkey,
Eur Arch Otorhinolaryngol. 2013 Nov;270(12):3183-6. doi: 10.1007/s00405-013-2539-4. Epub 2013 May 4.
Adequate disinfection level of the medical equipments should be maintained to prevent cross-contamination between patients. Otoscope specula are usually cleaned and disinfected appropriately after each use by disinfectant solutions. However, since otoscope heads are electrical instruments with irregular inner surface they may still harbor pathogenic microorganisms. According to manufacturers' instructions, otoscope heads can be cleaned externally with a damp cloth and they can be disinfected with aldehydes, tensides, and alcohols. Instrument heads should not be placed in liquids. Alcohols cannot be used on glass surfaces. How often an otoscope head must be cleaned to limit contamination is not well established. This study aimed to determine whether the otoscope heads harbor pathogenic microorganisms or not. A total of 53 otoscope heads were included in the study. Swab samples were obtained from the inner parts of the otoscope heads. For bacteriological examination, cotton swabs were inoculated onto 5 % sheep blood agar, chocolate agar, and eosine methylene blue agar plates. For fungal evaluation, cotton swabs were inoculated onto Sabouraud dextrose agars. Cultured microorganisms were evaluated macroscopically and microscopically. Of the 53 otoscope heads, 22 were found to be contaminated with bacteria and/or fungi. Eleven of them were colonized by one organism, 11 were colonized by more than one organism. Only one Pseudomonas species isolated as gram-negative microorganism. Gram-positive microorganisms were isolated from the remaining 19 samples. Staphylococcus species were the most common bacteria isolated. The most common fungal isolates were Aspergillus species. Two cultures were positive with Candida albicans. The results show that decontamination of the otoscope heads is usually ignored. However, they can harbor considerable amount of pathogenic microorganisms. The probability of contamination and the risk of cross-infection is high if they are used by otolaryngologists. In order to prevent cross-contamination between patients, guidelines indicating appropriate methods and frequency of cleaning and disinfection of otoscope heads needed to be described.
应保持医疗设备的充分消毒水平,以防止患者之间的交叉污染。耳镜探头通常在每次使用后用消毒剂溶液进行适当的清洁和消毒。然而,由于耳镜探头是具有不规则内表面的电气仪器,它们可能仍然存在致病微生物。根据制造商的说明,可以用湿布清洁耳镜探头的外部,也可以用醛类、表面活性剂和醇类对其进行消毒。探头不应放置在液体中。醇类不能用于玻璃表面。为了限制污染,耳镜探头需要清洁的频率尚不清楚。本研究旨在确定耳镜探头是否存在致病微生物。共有 53 个耳镜探头纳入研究。从耳镜探头的内部采集拭子样本。进行细菌学检查时,将棉签接种到 5%绵羊血琼脂、巧克力琼脂和伊红亚甲蓝琼脂平板上。进行真菌评估时,将棉签接种到沙氏葡萄糖琼脂上。培养的微生物通过宏观和微观进行评估。在 53 个耳镜探头中,有 22 个被细菌和/或真菌污染。其中 11 个被一种微生物定植,11 个被多种微生物定植。只有一种假单胞菌被分离为革兰氏阴性微生物。从其余 19 个样本中分离出革兰氏阳性微生物。分离出的最常见细菌为葡萄球菌属。最常见的真菌分离株为曲霉菌属。有两株培养物呈白色念珠菌阳性。结果表明,耳镜探头的去污处理通常被忽视。然而,它们可能含有大量的致病微生物。如果耳鼻喉科医生使用这些探头,污染的可能性和交叉感染的风险很高。为了防止患者之间的交叉污染,需要描述指示耳镜探头适当清洁和消毒方法及频率的指南。