Department of Molecular Biology, Ruđer Bošković Institute, Bijenička cesta 54, 10000, Zagreb, Croatia,
Biochem Genet. 2013 Oct;51(9-10):666-76. doi: 10.1007/s10528-013-9596-7. Epub 2013 May 5.
It is well known today that opine production anaerobic pathways are analogs to the classical glycolytic pathway (lactate production pathway). These pathways, catalyzed by a group of enzymes called opine dehydrogenases (OpDHs), ensure continuous flux of glycolysis and a constant supply of ATP by maintaining the NADH/NAD(+) ratio during exercise and hypoxia, thus regulating the cytosolic redox balance in glycolysis under anoxia. OpDHs are distributed in a wide range of marine invertebrate phyla, including sponges (Porifera). Phylogenetic analyses supported with enzymatic assays strongly indicate that sponge OpDHs constitute an enzyme class unrelated to other OpDHs. Therefore, OpDHs in marine invertebrates are divided into two groups, a mollusk/annelid type and a sponge type, which belongs to the OCD/mu-crystallin family.
如今众所周知，opine 产生的厌氧途径是经典糖酵解途径（乳酸产生途径）的类似物。这些途径由一组称为 opine 脱氢酶（OpDHs）的酶催化，通过在运动和缺氧期间维持 NADH/NAD（+）比，确保糖酵解的持续通量和 ATP 的持续供应，从而调节无氧条件下糖酵解中的细胞溶质氧化还原平衡。OpDHs 分布在广泛的海洋无脊椎动物门中，包括海绵（多孔动物门）。用酶促测定法支持的系统发育分析强烈表明，海绵 OpDHs 构成与其他 OpDHs 无关的酶类。因此，海洋无脊椎动物中的 OpDHs 分为两组，软体动物/环节动物类型和海绵类型，它们属于 OCD/mu-晶体蛋白家族。
