Chr. Hansen A/S, Discovery Department, 10-12 Bøge Allé, DK-2970 Hørsholm, Denmark.
University of Copenhagen, Faculty of Science, Department of Food Science, Rolighedsvej 30, DK-1958, Frederiksberg C, Denmark.
Int J Syst Evol Microbiol. 2013 Oct;63(Pt 10):3720-3726. doi: 10.1099/ijs.0.048769-0. Epub 2013 May 3.
Lactobacillus delbrueckii is divided into five subspecies based on phenotypic and genotypic differences. A novel isolate, designated ZN7a-9(T), was isolated from malted sorghum wort used for making an alcoholic beverage (dolo) in Burkina Faso. The results of 16S rRNA gene sequencing, DNA-DNA hybridization and peptidoglycan cell-wall structure type analyses indicated that it belongs to the species L. delbrueckii. The genome sequence of isolate ZN7a-9(T) was determined by Illumina-based sequencing. Multilocus sequence typing (MLST) and split-decomposition analyses were performed on seven concatenated housekeeping genes obtained from the genome sequence of strain ZN7a-9(T) together with 41 additional L. delbrueckii strains. The results of the MLST and split-decomposition analyses could not establish the exact subspecies of L. delbrueckii represented by strain ZN7a-9(T) as it clustered with L. delbrueckii strains unassigned to any of the recognized subspecies of L. delbrueckii. Strain ZN7a-9(T) additionally differed from the recognized type strains of the subspecies of L. delbrueckii with respect to its carbohydrate fermentation profile. In conclusion, the cumulative results indicate that strain ZN7a-9(T) represents a novel subspecies of L. delbrueckii closely related to Lactobacillus delbrueckii subsp. lactis and Lactobacillus delbrueckii subsp. delbrueckii for which the name Lactobacillus delbrueckii subsp. jakobsenii subsp. nov. is proposed. The type strain is ZN7a-9(T) = DSM 26046(T) = LMG 27067(T).
基于表型和基因型差异，德氏乳杆菌被分为五个亚种。从布基纳法索用于酿造酒精饮料（dolo）的麦芽高粱麦芽汁中分离到一株新型分离株，命名为ZN7a-9(T)。16S rRNA 基因测序、DNA-DNA 杂交和肽聚糖细胞壁结构型分析的结果表明，它属于德氏乳杆菌种。ZN7a-9(T)分离株的基因组序列通过基于 Illumina 的测序确定。对从 ZN7a-9(T) 菌株基因组获得的七个串联看家基因的 MLST 和分裂分解分析与 41 株额外的德氏乳杆菌菌株一起进行。MLST 和分裂分解分析的结果无法确定代表 ZN7a-9(T) 菌株的德氏乳杆菌的确切亚种，因为它与未分配到任何已识别的德氏乳杆菌亚种的德氏乳杆菌菌株聚类。ZN7a-9(T) 菌株与已识别的德氏乳杆菌亚种的模式菌株在碳水化合物发酵谱方面也存在差异。总之，累积结果表明，ZN7a-9(T) 菌株代表一种新型的德氏乳杆菌亚种，与 Lactobacillus delbrueckii subsp. lactis 和 Lactobacillus delbrueckii subsp. delbrueckii 密切相关，建议将其命名为 Lactobacillus delbrueckii subsp. jakobsenii subsp. nov.。模式菌株为 ZN7a-9(T) = DSM 26046(T) = LMG 27067(T)。
