Du Xin, Cao Kang, Tan Mingzheng, Pan Qu
Department of Pathogenic Biology, Chengdu Medical College, Chengdu, China.
Curr Microbiol. 2019 Feb;76(2):153-158. doi: 10.1007/s00284-018-1601-2. Epub 2018 Nov 24.
Strain CQ16Z1 was isolated from jamiecosley, a traditional Chinese pickle. The isolate was Gram-positive, non-motile, non-spore-forming, facultatively anaerobic, catalase-negative, and long rod-shaped. The optimal temperature for growth was 37 °C and the DNA G + C content was 39.1 mol%. The results of 16S rRNA and rpoA gene sequencing, DNA-DNA hybridization, and peptidoglycan type analyses indicated that strain CQ16Z1 belongs to the recognized species Lactobacillus futsaii. However, the analysis results of pheS gene sequencing, amplified fragment length polymorphism, phenotypic profiles, cellular fatty acid, cell-wall monosaccharide determination, and cell morphology revealed that the novel strain was obviously different from the type strain L. futsaii JCM17355, and had genetic relationship with Weissella cibaria to a certain degree, suggesting that the novel strain represents a novel subspecies of L. futsaii, for which the following names are proposed: L. futsaii subsp. futsaii subsp. nov. (type strain YM0097 = JCM 17355 = BCRC 80278) and L. futsaii subsp. chongqingii subsp.nov., with the type strain CQ16Z1 (= CCTCC AB2017187 = KCTC 21089).
菌株CQ16Z1是从中国传统泡菜“酱菜”中分离得到的。该分离菌株为革兰氏阳性，不运动，不产芽孢，兼性厌氧，过氧化氢酶阴性，呈长杆状。其生长的最适温度为37℃，DNA的G + C含量为39.1mol%。16S rRNA和rpoA基因测序、DNA-DNA杂交以及肽聚糖类型分析结果表明，菌株CQ16Z1属于已确认的福氏乳杆菌（Lactobacillus futsaii）物种。然而，pheS基因测序、扩增片段长度多态性、表型特征、细胞脂肪酸、细胞壁单糖测定以及细胞形态分析结果显示，该新菌株与福氏乳杆菌模式菌株JCM17355明显不同，且在一定程度上与食窦魏斯氏菌（Weissella cibaria）存在亲缘关系，这表明该新菌株代表福氏乳杆菌的一个新亚种，为此提出以下名称：福氏乳杆菌福氏亚种（L. futsaii subsp. futsaii subsp. nov.）（模式菌株YM0097 = JCM 17355 = BCRC 80278）和福氏乳杆菌重庆亚种（L. futsaii subsp. chongqingii subsp.nov.），模式菌株为CQ16Z1（= CCTCC AB2017187 = KCTC 21089）。
