Institute of Biotechnology, Zhejiang University, Hangzhou 310058, China.
J Zhejiang Univ Sci B. 2013 May;14(5):416-25. doi: 10.1631/jzus.B1200350.
To assess the short-term effect of scaling and root planing (SRP) and essential-oils mouthwash on the levels of specific bacteria in Chinese adults.
Fifty Chinese adults with chronic periodontitis were randomly assigned to full-mouth SRP or a 7-d essential-oils mouthwash regimen. In addition, 22 periodontally healthy adults used essential-oils mouthwash for 7 d. Clinical examination and plaque/saliva sampling were performed at baseline and on Day 7. Quantitative real-time polymerase chain reaction (PCR) was used to measure Aggregatibacter actinomycetemcomitans (Aa), Fusobacterium nucleatum (Fn), Porphyromonas gingivalis (Pg), Prevotella intermedia (Pi), and total bacterial loads in saliva, supra- and sub-gingival plaque samples.
The detection frequencies of four tested species remained unchanged after either treatment. However, the bacterial loads of Fn, Pg, and Pi were significantly reduced by SRP; the mean reduction of bacterial counts in saliva ranged from 52.2% to 62.5% (p<0.01), in supragingival plaque from 68.2% to 81.0% (p<0.05), and in subgingival plaque from 67.9% to 93.0% (p<0.01). Total bacterial loads were reduced after SRP in supra- and sub-gingival plaque (p<0.05). Essential-oils mouthwash reduced Fn levels in supragingival plaque by a mean of 53.2%, and reduced total bacterial loads in supra- and sub-gingival plaque (p<0.01). In subgingival plaque from periodontal patients, Pg and Pi reductions were high after SRP compared to essential-oils mouthwash (93.0% vs. 37.7% and 87.0% vs. 21.0%, p<0.05). No significant bacterial reduction was observed in periodontally healthy subjects using essential-oils mouthwash.
SRP and essential-oils mouthwash both have an impact on saliva and gingival plaque flora in Chinese periodontitis patients in 7 d, with greater microbiological improvement by SRP.
评估牙周洁治术（SRP）和精油漱口液对中国成年人特定细菌水平的短期影响。
将 50 名患有慢性牙周炎的中国成年人随机分为全口 SRP 组或 7 天精油漱口液组。此外，22 名牙周健康的成年人使用精油漱口液 7 天。在基线和第 7 天进行临床检查和菌斑/唾液采样。使用实时定量聚合酶链反应（PCR）测量唾液、龈上和龈下菌斑样本中伴放线聚集杆菌（Aa）、核梭杆菌（Fn）、牙龈卟啉单胞菌（Pg）、中间普氏菌（Pi）和总细菌负荷。
两种治疗方法后，四种检测物种的检出频率均无变化。然而，SRP 可显著降低 Fn、Pg 和 Pi 的细菌负荷；唾液中细菌计数的平均减少范围为 52.2%至 62.5%（p<0.01），龈上菌斑为 68.2%至 81.0%（p<0.05），龈下菌斑为 67.9%至 93.0%（p<0.01）。SRP 后龈上和龈下菌斑总细菌负荷减少（p<0.05）。精油漱口液可使龈上菌斑中 Fn 水平平均降低 53.2%，并降低龈上和龈下菌斑的总细菌负荷（p<0.01）。与使用精油漱口液相比，SRP 后牙周炎患者龈下菌斑中 Pg 和 Pi 的减少更为显著（93.0%比 37.7%和 87.0%比 21.0%，p<0.05）。牙周健康受试者使用精油漱口液未观察到明显的细菌减少。
SRP 和精油漱口液在 7 天内均对中国牙周炎患者的唾液和牙龈菌斑菌群产生影响，SRP 具有更大的微生物改善作用。
