Department of Orthopaedics, Ninth People's Hospital, Medical School of Shanghai Jiao Tong University.
Artif Organs. 2013 Oct;37(10):884-93. doi: 10.1111/aor.12092. Epub 2013 May 6.
We aimed to introduce an in vivo bioreactor-vascular pedicle threaded through the central portion of a scaffold in which a vascularized bone graft was prefabricated using adenoviral human BMP-2 gene (AdBMP2)-modified bone marrow mesenchymal stem cells (BMSCs), beta tricalcium phosphate (β-TCP), a vessel bundle, and muscularis membrane(group A). As controls, Adβgal-BMSCs/β-TCP granules, vessel bundle, and the muscularis membrane (group B); BMSCs/β-TCP granules, vessel bundle, and muscularis membrane (group C); and β-TCP granules, vessel bundle, and muscularis membrane (group D) were prepared. Formation of bone tissue and a vascular network was assessed by microangiography and histological methods 4 weeks after prefabrication. New cartilage and bone tissue in the space between β-TCP granules (mainly endochondral bone) were confirmed by histology, and a de novo vascular network circulating from the vessel bundle through newly formed bone tissue was observed in group A. Formation of bone or cartilage was not observed in the control groups. We concluded that the in vivo bioreactor is a promising method for prefabrication of vascularized functional bone.
我们旨在介绍一种体内生物反应器，该生物反应器通过支架的中央部分穿过血管蒂，其中使用腺病毒人 BMP-2 基因（AdBMP2）修饰的骨髓间充质干细胞（BMSCs）、β-磷酸三钙（β-TCP）、血管束和肌膜预制了血管化骨移植物（A 组）。作为对照，制备了 Adβgal-BMSCs/β-TCP 颗粒、血管束和肌膜（B 组）；BMSCs/β-TCP 颗粒、血管束和肌膜（C 组）；和β-TCP 颗粒、血管束和肌膜（D 组）。预制后 4 周，通过微血管造影和组织学方法评估骨组织和血管网络的形成。通过组织学证实了β-TCP 颗粒之间（主要是软骨内骨）新的软骨和骨组织的形成，并且在 A 组中观察到从血管束穿过新形成的骨组织循环的新血管网络。在对照组中未观察到骨或软骨的形成。我们得出结论，体内生物反应器是预制血管化功能性骨的有前途的方法。
