Integrated Prosthetics and Reconstruction, Department of Head and Neck Surgery, Chris O'Brien Lifehouse, Camperdown, NSW 2050, Australia.
Central Clinical School, Faculty of Medicine and Health, The University of Sydney, Camperdown, NSW 2050, Australia.
Cells. 2023 Jun 27;12(13):1724. doi: 10.3390/cells12131724.
Periosteum is a highly vascularized membrane lining the surface of bones. It plays essential roles in bone repair following injury and reconstruction following invasive surgeries. To broaden the use of periosteum, including for augmenting in vitro bone engineering and/or in vivo bone repair, we have developed an ex vivo perfusion bioreactor system to maintain the cellular viability and metabolism of surgically resected periosteal flaps. Each specimen was placed in a 3D printed bioreactor connected to a peristaltic pump designed for the optimal flow rates of tissue perfusate. Nutrients and oxygen were perfused via the periosteal arteries to mimic physiological conditions. Biochemical assays and histological staining indicate component cell viability after perfusion for almost 4 weeks. Our work provides the proof-of-concept of ex vivo periosteum perfusion for long-term tissue preservation, paving the way for innovative bone engineering approaches that use autotransplanted periosteum to enhance in vivo bone repair.
骨膜是一种高度血管化的膜，覆盖在骨骼表面。它在受伤后的骨修复和侵袭性手术后的重建中发挥着重要作用。为了拓宽骨膜的用途，包括用于体外骨工程和/或体内骨修复，我们开发了一种离体灌注生物反应器系统，以维持手术切除的骨膜瓣的细胞活力和代谢。每个标本都放置在一个 3D 打印的生物反应器中，该生物反应器与蠕动泵相连，以实现组织灌流最佳流速。营养物质和氧气通过骨膜动脉灌注，以模拟生理条件。生化分析和组织学染色表明，在灌注近 4 周后，细胞仍具有活力。我们的工作为离体骨膜灌注进行长期组织保存提供了概念验证，为使用自体移植骨膜增强体内骨修复的创新骨工程方法铺平了道路。
