Department of Gynecology & Obstetrics, Hvidovre University Hospital, Copenhagen, Denmark.
Acta Obstet Gynecol Scand. 2013 Sep;92(9):1032-40. doi: 10.1111/aogs.12162. Epub 2013 Jun 1.
A study was undertaken to assess the distribution of high-risk HPV-genotypes in high-grade cervical intraepithelial neoplastic lesions in Danish women.
Observational, cross-sectional.
Danish data from a multi-centre study undertaken in 13 European countries.
290 archived fixed biopsies with high-grade cervical lesions from the Departments of Pathology at the University Hospitals in Hvidovre and Odense, Denmark.
Relevant histological samples were anonymized and shipped to a central laboratory for histopathology review and PCR-testing for HPV-DNA. A standardised HPV-test methodology was utilised to enable comparison of HPV-genotype distribution.
Of 290 Danish cervical samples, 276 were evaluated as histologically adequate and all of these were HPV-positive (HPV⁺). Of the HPV⁺ samples 77.9% were diagnosed with a single HPV-type, with cervical intraepithelial neoplasia (CIN)3 diagnosed in 82.3% and CIN2, CIN2/3, adenocarcinoma in situ (AIS) and AIS⁺ other high-grade lesion diagnosed in the remaining 17.7%. The most prevalent HPV-types were: HPV16 (54.0%), HPV33 (13.5%), HPV31 (10.7%), HPV18 (7.9%) and HPV52 (4.7%). Of the HPV⁺ samples, 21.4% were diagnosed with multiple HPV-types, with CIN3 diagnosed in 79.6% and CIN2, CIN2/3, AIS and AIS⁺ other high-grade lesion diagnosed in the remaining 20.4%. The most prevalent HPV-types were: HPV16 (49.2%), HPV31 (30.5%), HPV52 (27.1%), HPV51 (20.3%), HPV18 (16.9%), HPV33 (13.6%), HPV45 (11.9%), with 0.7% unknown types.
HPV16 and HPV18 were detected in approximately 75% of high-grade intraepithelial cervical lesions in a Danish population (single or multiple infections); these two genotypes are considered causative in at least 61.9% of the high-grade intraepithelial lesions (single infection).
本研究旨在评估高危型 HPV 基因型在丹麦女性高级别宫颈上皮内瘤变中的分布情况。
观察性、横断面研究。
丹麦 13 个国家多中心研究的数据。
丹麦 290 例存档的高级别宫颈病变固定活检标本，来自丹麦 Hvidovre 和 Odense 大学医院的病理学系。
对相关组织学样本进行匿名处理，并运送到一个中央实验室进行组织病理学复查和 HPV-DNA 的 PCR 检测。采用标准化 HPV 检测方法，以实现 HPV 基因型分布的比较。
在 290 例丹麦宫颈样本中，276 例组织学评估充分，且均为 HPV 阳性（HPV⁺）。在 HPV⁺样本中，77.9% 诊断为单一 HPV 型，其中 82.3% 诊断为宫颈上皮内瘤变（CIN）3，其余 17.7% 诊断为 CIN2、CIN2/3、原位腺癌（AIS）和 AIS⁺其他高级别病变。最常见的 HPV 型为：HPV16（54.0%）、HPV33（13.5%）、HPV31（10.7%）、HPV18（7.9%）和 HPV52（4.7%）。在 HPV⁺样本中，21.4% 诊断为多种 HPV 型，其中 79.6% 诊断为 CIN3，其余 20.4% 诊断为 CIN2、CIN2/3、AIS 和 AIS⁺其他高级别病变。最常见的 HPV 型为：HPV16（49.2%）、HPV31（30.5%）、HPV52（27.1%）、HPV51（20.3%）、HPV18（16.9%）、HPV33（13.6%）、HPV45（11.9%），另有 0.7% 为未知类型。
在丹麦人群中，HPV16 和 HPV18 检测出存在于约 75%的高级别宫颈上皮内病变（单一或多重感染）中；这两种基因型被认为至少与 61.9%的高级别上皮内病变（单一感染）有关。
