Cui H
People's Hospital, Beijing Medical University.
Zhonghua Fu Chan Ke Za Zhi. 1990 Mar;25(2):80-3, 123.
HMD4, a human IgG (lambda) monoclonal antibody against ovarian carcinoma, was purified by affinity chromatography of CNBr-activated Sepharose 4 B coupled with goat antihuman IgG, 131I-HMD4 was prepared by chloramin-T or Iodogen method. The nude mice bearing human ovarian carcinoma (OSC-2) were intraperitoneally injected with 131I-HMD4, while 131I-labeled normal human IgG and normal murine IgG were used as controls. Imaging was performed daily using a gamma camera. For analysis of distribution and metabolism of 131I-HND4 in the nude mouse models, T/NT and T/B ratios were calculated. The HMD4 was specifically localized at the tumor masses in nude mice and the best imaging was obtained at 72 hours after injection. Total positive rate of imaging was 82%, while negative rate in the controls was 91%. Radioimmunoimaging index was highest (4.17) at 48 hours after injection. The average half life of 131I-HMD4 in the blood was about 30 hours.
HMD4是一种抗卵巢癌的人IgG（λ）单克隆抗体，通过用偶联山羊抗人IgG的溴化氰活化琼脂糖4B进行亲和层析纯化，用氯胺-T或碘代法制备131I-HMD4。将荷人卵巢癌（OSC-2）裸鼠腹腔注射131I-HMD4，同时用131I标记的正常人IgG和正常鼠IgG作为对照。每天用γ相机进行显像。为分析131I-HND4在裸鼠模型中的分布和代谢，计算T/NT和T/B比值。HMD4特异性定位于裸鼠肿瘤块，注射后72小时显像效果最佳。显像总阳性率为82%，而对照组阴性率为91%。注射后48小时放射免疫显像指数最高（4.17）。131I-HMD4在血液中的平均半衰期约为30小时。
