College of Life Science and Technology, Heilongjiang Bayi Agricultural University, Daqing 163319, PR China.
Bioresour Technol. 2013 Jul;139:113-9. doi: 10.1016/j.biortech.2013.04.033. Epub 2013 Apr 13.
A lignin degradation bacterial consortium named LDC was screened from the sludge of a reeds pond by a restricted subculture. It could break down 60.9% lignin in reeds at 30°C under conditions of static culture within 15 days. In order to analyze the diversity of LDC, plate isolation, 16S rDNA clone library and ARDRA (Amplified Ribosomal DNA Restriction Analysis) were performed. Six bacterial strains were isolated from LDC and eighteen DNA phylotypes were identified from 230 bacterial analyzed clones. They were classified into Clostridiales(9.1%), Geovibrio thiophilus (5.1%), Desulfomicrobium (10.9%), Pseudomonas sp. (25.2%), Azoarcus sp. (5.1%), Thauera (5.1%), Paenibacillus sp. (5.1%), Cohnella sp. (2.2%), Acinetobacter sp. (3.1%), Microbacterium (7.8%), and uncultured bacterium (21.3%). In addition, physical characteristics of paper hand-sheets between biological pretreatment and chemical pretreatment were compared. The results showed that LDC had the capability of lignin degradation and was efficient for pulping, which would provide a new choice for biopulping.
从芦苇池塘的污泥中通过限制亚培养筛选出一种名为 LDC 的木质素降解细菌群落。在 30°C 下静置培养 15 天的条件下，它可以分解芦苇中的 60.9%木质素。为了分析 LDC 的多样性，进行了平板分离、16S rDNA 克隆文库和ARDRA（扩增核糖体 DNA 限制性分析）。从 LDC 中分离出 6 株细菌，从 230 株分析克隆中鉴定出 18 种 DNA 基因型。它们被分类为梭菌目（9.1%）、脱硫微菌属（10.9%）、假单胞菌属（25.2%）、节杆菌属（5.1%）、固氮菌属（5.1%）、陶厄氏菌属（5.1%）、芽孢杆菌属（5.1%）、科恩氏菌属（2.2%）、不动杆菌属（3.1%）、微杆菌属（7.8%）和未培养细菌（21.3%）。此外，还比较了生物预处理和化学预处理之间的手抄纸物理特性。结果表明，LDC 具有木质素降解能力，并且对制浆有效，这将为生物制浆提供新的选择。
