State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, 214122, China.
Environ Sci Pollut Res Int. 2013 Dec;20(12):8484-90. doi: 10.1007/s11356-013-1771-1. Epub 2013 May 7.
Bisphenol A (BPA), which is ubiquitous in the environment, is an example of an endocrine-disrupting compound (EDC). Ammonium assimilation has an important function in plant growth and development. However, insufficient information on the potential effect of BPA on ammonium assimilation in plants is available. In this study, the effects of BPA on ammonium assimilation in roots of soybean seedlings were investigated. During the stress period, 1.5 mg L(-1) of BPA improved glutamine synthetase (GS)/glutamate synthase (GOGAT) cycle and glutamate dehydrogenase (GDH) pathway in ammonium assimilation. The amino acid and the soluble protein contents increased in the soybeans. At 17.2 and 50.0 mg L(-1) of BPA, the GS/GOGAT cycle was inhibited and the GDH pathway was promoted. The amino acid content increased and the soluble protein content decreased. During the recovery period, the GS/GOGAT cycle and the GDH pathway recovered at 1.5 and 17.2 mg L(-1) of BPA but not at 50.0 mg L(-1) of BPA. The amino acid content continuously increased and the soluble protein content decreased compared with those in the control treatment. In summary, BPA treatment could affect the contents of soluble protein and amino acid in the soybean roots by regulating ammonium assimilation.
双酚 A（BPA）是环境中普遍存在的一种内分泌干扰化合物（EDC）。铵同化在植物生长发育中具有重要功能。然而，关于 BPA 对植物铵同化潜在影响的信息还不够充分。在这项研究中，研究了 BPA 对大豆幼苗根系铵同化的影响。在胁迫期，1.5mg/L 的 BPA 提高了谷氨酰胺合成酶（GS）/谷氨酸合酶（GOGAT）循环和谷氨酸脱氢酶（GDH）途径中的铵同化。大豆中的氨基酸和可溶性蛋白含量增加。在 17.2 和 50.0mg/L 的 BPA 下，GS/GOGAT 循环受到抑制，GDH 途径得到促进。氨基酸含量增加，可溶性蛋白含量减少。在恢复期，1.5 和 17.2mg/L 的 BPA 恢复了 GS/GOGAT 循环和 GDH 途径，但 50.0mg/L 的 BPA 没有恢复。与对照处理相比，氨基酸含量持续增加，可溶性蛋白含量下降。总之，BPA 处理可以通过调节铵同化来影响大豆根系中可溶性蛋白和氨基酸的含量。
