Simultaneous assay for ten bacteria and toxins in spiked clinical samples using a microflow cytometer.

Bacterial infection and intoxication can present with common symptoms. The ability to identify a bacteria or toxin rapidly in clinical samples is critical for administering the appropriate treatment. The microflow cytometer has previously demonstrated the ability to test for six bacteria and toxins simultaneously in buffer. In this study, the number of bacteria and toxins analyzed was increased to ten, positive and negative controls were incorporated in all assays, and most importantly, multiplexed immunoassays were demonstrated in clinical matrices. The multiplexed assays using the microflow cytometer demonstrated detection limits similar to or better than other reported antibody-based methods for pathogen detection (ELISA, lateral flow, array biosensors). In most cases, detection from complex clinical matrices (serum and nasal wash) achieved limits of detection equivalent to those for spiked buffer samples. Clinical samples spiked with bacteria and/or toxins were also analyzed successfully in blind trials.