Dental College, Pontifical Catholic University of Rio Grande do Sul, Hospital São Lucas, Porto Alegre, RS, Brazil.
Head Neck. 2014 Jan;36(1):84-93. doi: 10.1002/hed.23260. Epub 2013 May 4.
The purpose of this study was to investigate the influence of diabetes and corticotherapy on the development of osteonecrosis of the jaws associated with sodium alendronate.
Rats were allocated into 4 groups of 11 animals each, representing different treatments: (1) alendronate; (2) alendronate and corticotherapy; (3) alendronate and diabetes; and (4) control. Tooth extractions were performed in all animals, and histological analysis was performed by hematoxylin and eosin staining and immunohistochemistry using anti-bone morphogenetic protein (BMP)-4 and anti-matrix metalloproteinase (MMP)-13 antibodies.
On hematoxylin and eosin analysis, proportions of inflammatory infiltrate, microbial colonies, and osteonecrosis were significantly greater in the diabetes group. BMP-4 expression in connective tissue was higher in the corticosteroid group than the alendronate group. There were no significant differences between the other groups. MMP-13 expression did not differ between the groups analyzed.
Diabetes but not corticotherapy is associated with jaw osteonecrosis in rats undergoing alendronate therapy and subjected to tooth extractions.
本研究旨在探讨糖尿病和皮质激素治疗对接受阿仑膦酸钠治疗并接受拔牙的大鼠颌骨骨坏死发展的影响。
将大鼠分为 4 组，每组 11 只，分别代表不同的治疗方法：（1）阿仑膦酸钠；（2）阿仑膦酸钠和皮质激素治疗；（3）阿仑膦酸钠和糖尿病；和（4）对照组。所有动物均进行拔牙，通过苏木精和伊红染色和抗骨形态发生蛋白（BMP）-4 和基质金属蛋白酶（MMP）-13 抗体的免疫组织化学分析进行组织学分析。
在苏木精和伊红分析中，糖尿病组的炎症浸润、微生物菌落和骨坏死的比例明显更高。与阿仑膦酸钠组相比，皮质激素组结缔组织中 BMP-4 的表达更高。其他组之间没有显着差异。分析的各组之间 MMP-13 表达没有差异。
在接受阿仑膦酸钠治疗并接受拔牙的大鼠中，糖尿病而非皮质激素治疗与颌骨骨坏死有关。
