Edwardson J M, Daniels-Holgate P U
Department of Pharmacology, University of Cambridge, UK.
FEBS Lett. 1990 Jun 4;265(1-2):107-9. doi: 10.1016/0014-5793(90)80895-p.
We have previously described the reconstitution, in a cell-free system, of the constitutive delivery of a newly synthesized protein, influenza neuraminidase, to the plasma membrane in BHK cells. Here we report some of the characteristics of this in vitro membrane fusion event. We show that fusion requires ATP hydrolysis, and exploit this requirement to distinguish the time-course of fusion from that of neuraminidase action. In addition, we present evidence for the occurrence of multiple fusions between hybrid membrane vesicles.
我们之前曾描述过，在无细胞体系中，新合成的蛋白质流感病毒神经氨酸酶在BHK细胞中组成型转运至质膜的过程。在此我们报告这一体外膜融合事件的一些特征。我们发现融合需要ATP水解，并利用这一需求来区分融合的时间进程与神经氨酸酶作用的时间进程。此外，我们提供了杂交膜囊泡之间发生多次融合的证据。
