Weiler R, Meyerson G, Fischer-Colbrie R, Laslop A, Påhlman S, Floor E, Winkler H
Department of Pharmacology, University of Innsbruck, Austria.
FEBS Lett. 1990 Jun 4;265(1-2):27-9. doi: 10.1016/0014-5793(90)80875-j.
Human neuroblastoma cells were cultured either in the absence or presence of 12-O-tetradecanoylphorbol-13-acetate (TPA) known to induce neuronal differentiation. This treatment led to a marked increase in the concentration of secretogranin II but to a decrease of chromogranin A. Analogous changes were observed for the respective mRNAs. Thus during differentiation of these cells the biosynthesis of two vesicle constituents of large dense core vesicles is differentially regulated as determined both at the mRNA and the protein level. Levels of both synaptin/synaptophysin large dense core vesicles is differentially regulated as determined both at the mRNA and the protein level. Levels of both synaptin/synaptophysin and SV2 were also elevated but to a smaller degree than that of secretogranin II.
人神经母细胞瘤细胞在不存在或存在已知可诱导神经元分化的12 - O - 十四烷酰佛波醇 - 13 - 乙酸酯（TPA）的情况下进行培养。这种处理导致分泌粒蛋白II的浓度显著增加，但嗜铬粒蛋白A的浓度降低。在各自的mRNA中也观察到类似的变化。因此，在这些细胞的分化过程中，大致密核心囊泡的两种囊泡成分的生物合成在mRNA和蛋白质水平上均受到差异调节。突触结合蛋白/突触小泡蛋白大致密核心囊泡的水平在mRNA和蛋白质水平上均受到差异调节。突触结合蛋白/突触小泡蛋白和SV2的水平也升高，但程度小于分泌粒蛋白II。
