Höfle G, Weiler R, Fischer-Colbrie R, Humpel C, Laslop A, Wohlfarter T, Hogue-Angeletti R, Saria A, Fleming P J, Winkler H
Department of Pharmacology, University of Innsbruck, Austria.
Regul Pept. 1991 Feb 26;32(3):321-31. doi: 10.1016/0167-0115(91)90025-c.
The levels of various components of chromaffin granules were determined in rat adrenals after treatment with several stimulants. After reserpine the levels of calcitonin gene-related peptide (CGRP), neuropeptide Y (NPY) and chromogranin B but not those of chromogranin A and secretogranin II were elevated. On the other hand, the mRNA of chromogranins A, B and secretogranin II were significantly increased. Treatment with oxotremorine or nicotine (multiple injections for 2 or 3 days) induced analogous changes for peptide and mRNA levels, however, the increases were smaller and for the mRNA less consistent. A single injection of oxotremorine or nicotine raised only the levels of CGRP and NPY and of the NPY mRNA whereas those of the chromogranins and their respective mRNAs remained unaltered. Amongst the membrane proteins only the levels of dopamine beta-hydroxylase are increased after prolonged stimulation, whereas those of cytochrome b-561, carboxypeptidase H and synaptin/synaptophysin (SYN) remain unaltered. Thus, the biosynthesis of chromaffin granules can be regulated in quite sophisticated patterns.
在用几种刺激剂处理大鼠肾上腺后，测定了嗜铬颗粒各种成分的水平。利血平处理后，降钙素基因相关肽（CGRP）、神经肽Y（NPY）和嗜铬粒蛋白B的水平升高，但嗜铬粒蛋白A和分泌粒蛋白II的水平未升高。另一方面，嗜铬粒蛋白A、B和分泌粒蛋白II的mRNA显著增加。用氧化震颤素或尼古丁处理（多次注射2或3天）可诱导肽和mRNA水平发生类似变化，然而，增加幅度较小，且mRNA的变化不太一致。单次注射氧化震颤素或尼古丁仅提高了CGRP和NPY的水平以及NPY mRNA的水平，而嗜铬粒蛋白及其各自mRNA的水平保持不变。在膜蛋白中，只有多巴胺β-羟化酶的水平在长期刺激后升高，而细胞色素b-561、羧肽酶H和突触素/突触小泡蛋白（SYN）的水平保持不变。因此，嗜铬颗粒的生物合成可以以相当复杂的模式进行调节。
