• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

白细胞介素-2 激活 CTLL-2 T 淋巴细胞过程中依赖二酰基甘油激酶形成的磷脂酸分子种类。

Diacylglycerol kinase-dependent formation of phosphatidic acid molecular species during interleukin-2 activation in CTLL-2 T-lymphocytes.

机构信息

Department of Chemistry, Graduate School of Science, Chiba University, 1-33 Yayoi-cho, Inage-ku, Chiba 263-8522, Japan.

出版信息

FEBS Open Bio. 2012 Sep 4;2:267-72. doi: 10.1016/j.fob.2012.08.006. Print 2012.

DOI:10.1016/j.fob.2012.08.006
PMID:23650609
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3642166/
Abstract

Although effective liquid chromatography (LC)/mass spectrometry (MS) methods enabling the separation of phospholipid molecular species have been developed, there are still problems with an intracellular signaling molecule, phosphatidic acid (PA). In this study, we optimized LC/MS conditions to improve the quantitative detection of PA molecular species from a cellular lipid mixture. Using the newly developed LC/MS method, we showed that stimulation of CTLL-2 murine T-lymphocytes by interleukin-2 (IL-2) induced a significant increase of 36:1-, 36:2-, 40:5- and 40:6-diacyl-PA. A diacylglycerol kinase (DGK) inhibitor, R59949, attenuated the increase of 36:1-, 40:5-, 40:6-diacyl-PA, suggesting that DGK IL-2-dependently and selectively generated these diacyl-PA species.

摘要

虽然已经开发出了有效的液相色谱（LC）/质谱（MS）方法来分离磷脂分子物种，但对于细胞内信号分子磷脂酸（PA）仍然存在问题。在这项研究中，我们优化了 LC/MS 条件，以提高从细胞脂质混合物中定量检测 PA 分子物种的能力。使用新开发的 LC/MS 方法，我们表明白细胞介素-2（IL-2）刺激 CTLL-2 小鼠 T 淋巴细胞会显著增加 36:1-、36:2-、40:5-和 40:6-二酰基-PA。二酰基甘油激酶（DGK）抑制剂 R59949 减弱了 36:1-、40:5-、40:6-二酰基-PA 的增加，表明 DGK 依赖于 IL-2 并选择性地产生这些二酰基-PA 物种。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/66f8/3642166/edb91455641f/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/66f8/3642166/4fc1155c0af7/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/66f8/3642166/ba7a4b88b026/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/66f8/3642166/0f0b6396edab/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/66f8/3642166/edb91455641f/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/66f8/3642166/4fc1155c0af7/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/66f8/3642166/ba7a4b88b026/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/66f8/3642166/0f0b6396edab/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/66f8/3642166/edb91455641f/gr4.jpg

相似文献

1
Diacylglycerol kinase-dependent formation of phosphatidic acid molecular species during interleukin-2 activation in CTLL-2 T-lymphocytes.白细胞介素-2 激活 CTLL-2 T 淋巴细胞过程中依赖二酰基甘油激酶形成的磷脂酸分子种类。
FEBS Open Bio. 2012 Sep 4;2:267-72. doi: 10.1016/j.fob.2012.08.006. Print 2012.
2
Interleukin-2 causes an increase in saturated/monounsaturated phosphatidic acid derived from 1,2-diacylglycerol and 1-O-alkyl-2-acylglycerol.白细胞介素-2会导致源自1,2-二酰基甘油和1-O-烷基-2-酰基甘油的饱和/单不饱和磷脂酸增加。
J Biol Chem. 1999 Jun 11;274(24):16846-52. doi: 10.1074/jbc.274.24.16846.
3
Diacylglycerol kinase ζ generates dipalmitoyl-phosphatidic acid species during neuroblastoma cell differentiation.二酰基甘油激酶ζ在神经母细胞瘤细胞分化过程中产生二棕榈酰磷脂酸物种。
Biochem Biophys Rep. 2016 Oct 15;8:352-359. doi: 10.1016/j.bbrep.2016.10.004. eCollection 2016 Dec.
4
Cellular phosphatidic acid sensor, α-synuclein N-terminal domain, detects endogenous phosphatidic acid in macrophagic phagosomes and neuronal growth cones.细胞磷脂酸传感器α-突触核蛋白N端结构域可检测巨噬细胞吞噬体和神经元生长锥中的内源性磷脂酸。
Biochem Biophys Rep. 2020 May 20;22:100769. doi: 10.1016/j.bbrep.2020.100769. eCollection 2020 Jul.
5
Where do substrates of diacylglycerol kinases come from? Diacylglycerol kinases utilize diacylglycerol species supplied from phosphatidylinositol turnover-independent pathways.二酰基甘油激酶的底物从何而来？二酰基甘油激酶利用由不依赖磷脂酰肌醇周转途径提供的二酰基甘油种类。
Adv Biol Regul. 2018 Jan;67:101-108. doi: 10.1016/j.jbior.2017.09.003. Epub 2017 Sep 9.
6
Substrate preference of stress-activated phospholipase D in Chlamydomonas and its contribution to PA formation.衣藻中应激激活磷脂酶D的底物偏好及其对磷脂酸形成的贡献。
Plant J. 2003 Jun;34(5):595-604. doi: 10.1046/j.1365-313x.2003.01750.x.
7
New Era of Diacylglycerol Kinase, Phosphatidic Acid and Phosphatidic Acid-Binding Protein.二酰基甘油激酶、磷酸脂酸和磷酸脂酸结合蛋白的新纪元。
Int J Mol Sci. 2020 Sep 16;21(18):6794. doi: 10.3390/ijms21186794.
8
Palmitic acid- and/or palmitoleic acid-containing phosphatidic acids are generated by diacylglycerol kinase α in starved Jurkat T cells.饥饿的 Jurkat T 细胞中，二酰基甘油激酶 α 产生含有棕榈酸和/或棕榈油酸的磷脂酸。
Biochem Biophys Res Commun. 2020 May 14;525(4):1054-1060. doi: 10.1016/j.bbrc.2020.02.162. Epub 2020 Mar 14.
9
Regulation of interleukin-2 responses by phosphatidic acid.磷脂酸对白细胞介素-2反应的调节
Eur J Immunol. 1992 Jul;22(7):1883-9. doi: 10.1002/eji.1830220731.
10
Development of a reverse-phase liquid chromatography electrospray ionization mass spectrometry method for lipidomics, improving detection of phosphatidic acid and phosphatidylserine.一种用于脂质组学的反相液相色谱电喷雾电离质谱方法的开发，改进磷脂酸和磷脂酰丝氨酸的检测。
Anal Biochem. 2008 Apr 1;375(1):124-31. doi: 10.1016/j.ab.2007.12.027. Epub 2007 Dec 25.

引用本文的文献

1
New Era of Diacylglycerol Kinase, Phosphatidic Acid and Phosphatidic Acid-Binding Protein.二酰基甘油激酶、磷酸脂酸和磷酸脂酸结合蛋白的新纪元。
Int J Mol Sci. 2020 Sep 16;21(18):6794. doi: 10.3390/ijms21186794.
2
Diacylglycerol kinase δ and sphingomyelin synthase-related protein functionally interact via their sterile α motif domains.二酰基甘油激酶 δ 和鞘磷脂合酶相关蛋白通过其无活性α基序结构域发生功能相互作用。
J Biol Chem. 2020 Mar 6;295(10):2932-2947. doi: 10.1074/jbc.RA119.012369. Epub 2020 Jan 24.
3
Microarray analysis of gene expression in the diacylglycerol kinase η knockout mouse brain.

本文引用的文献

1
Diacylglycerol kinase alpha enhances hepatocellular carcinoma progression by activation of Ras-Raf-MEK-ERK pathway.二酰基甘油激酶 α 通过激活 Ras-Raf-MEK-ERK 通路促进肝癌进展。
J Hepatol. 2012 Jul;57(1):77-83. doi: 10.1016/j.jhep.2012.02.026. Epub 2012 Mar 14.
2
Understanding of the roles of phospholipase D and phosphatidic acid through their binding partners.通过其结合伴侣来理解磷脂酶 D 和磷脂酸的作用。
Prog Lipid Res. 2012 Apr;51(2):71-81. doi: 10.1016/j.plipres.2011.12.003. Epub 2011 Dec 28.
3
Regulation and functions of diacylglycerol kinases.
二酰基甘油激酶η基因敲除小鼠大脑中基因表达的微阵列分析。
Biochem Biophys Rep. 2019 Jun 25;19:100660. doi: 10.1016/j.bbrep.2019.100660. eCollection 2019 Sep.
4
Diacylglycerol kinase ζ generates dipalmitoyl-phosphatidic acid species during neuroblastoma cell differentiation.二酰基甘油激酶ζ在神经母细胞瘤细胞分化过程中产生二棕榈酰磷脂酸物种。
Biochem Biophys Rep. 2016 Oct 15;8:352-359. doi: 10.1016/j.bbrep.2016.10.004. eCollection 2016 Dec.
5
Activation of conventional and novel protein kinase C isozymes by different diacylglycerol molecular species.不同二酰基甘油分子种类对传统和新型蛋白激酶C同工酶的激活作用。
Biochem Biophys Rep. 2016 Jul 20;7:361-366. doi: 10.1016/j.bbrep.2016.07.017. eCollection 2016 Sep.
6
A novel diacylglycerol kinase α-selective inhibitor, CU-3, induces cancer cell apoptosis and enhances immune response.一种新型二酰基甘油激酶α选择性抑制剂CU-3可诱导癌细胞凋亡并增强免疫反应。
J Lipid Res. 2016 Mar;57(3):368-79. doi: 10.1194/jlr.M062794. Epub 2016 Jan 14.
7
Diacylglycerol kinase δ phosphorylates phosphatidylcholine-specific phospholipase C-dependent, palmitic acid-containing diacylglycerol species in response to high glucose levels.二酰甘油激酶δ在高葡萄糖水平刺激下，使磷脂酰胆碱特异性磷脂酶C依赖性、含棕榈酸的二酰甘油物种发生磷酸化。
J Biol Chem. 2014 Sep 19;289(38):26607-26617. doi: 10.1074/jbc.M114.590950. Epub 2014 Aug 11.
二酰基甘油激酶的调控与功能
Chem Rev. 2011 Oct 12;111(10):6186-208. doi: 10.1021/cr1004106. Epub 2011 Jul 29.
4
Effect of D-allose on prostate cancer cell lines: phospholipid profiling by nanoflow liquid chromatography-tandem mass spectrometry.D-allo 果糖对前列腺癌细胞系的影响：纳米流液相色谱-串联质谱法的磷脂谱分析。
Anal Bioanal Chem. 2011 Aug;401(2):689-98. doi: 10.1007/s00216-011-5113-1. Epub 2011 Jun 3.
5
Characterization of substrate preference for Slc1p and Cst26p in Saccharomyces cerevisiae using lipidomic approaches and an LPAAT activity assay.采用脂质组学方法和 LPAAT 活性测定法对酿酒酵母 Slc1p 和 Cst26p 的底物偏好性进行表征。
PLoS One. 2010 Aug 4;5(8):e11956. doi: 10.1371/journal.pone.0011956.
6
Diacylglycerol kinases as emerging potential drug targets for a variety of diseases.二酰基甘油激酶作为多种疾病新出现的潜在药物靶点。
Curr Drug Targets. 2008 Aug;9(8):626-40. doi: 10.2174/138945008785132394.
7
Diacylglycerol kinases: at the hub of cell signalling.二酰基甘油激酶：处于细胞信号传导的核心位置。
Biochem J. 2008 Jan 1;409(1):1-18. doi: 10.1042/BJ20071040.
8
Quantitative analysis of phospholipids by thin-layer chromatography and phosphorus analysis of spots.通过薄层色谱法对磷脂进行定量分析以及对斑点进行磷分析。
Lipids. 1966 Jan;1(1):85-6. doi: 10.1007/BF02668129.
9
Diacylglycerol kinases: why so many of them?二酰基甘油激酶：为何有这么多种？
Biochim Biophys Acta. 2007 Jul;1771(7):793-806. doi: 10.1016/j.bbalip.2007.04.006. Epub 2007 Apr 14.
10
Diacylglycerol kinase alpha suppresses tumor necrosis factor-alpha-induced apoptosis of human melanoma cells through NF-kappaB activation.二酰甘油激酶α通过激活核因子κB抑制肿瘤坏死因子α诱导的人黑素瘤细胞凋亡。
Biochim Biophys Acta. 2007 Apr;1771(4):462-74. doi: 10.1016/j.bbalip.2006.12.008. Epub 2007 Jan 8.