高分辨率分析α-珠蛋白基因座上的顺式作用调控网络。
High-resolution analysis of cis-acting regulatory networks at the α-globin locus.
机构信息
MRC Molecular Haematology Unit, Weatherall Institute of Molecular Medicine, Oxford University, Oxford OX3 9DS, UK.
出版信息
Philos Trans R Soc Lond B Biol Sci. 2013 May 6;368(1620):20120361. doi: 10.1098/rstb.2012.0361. Print 2013.
Abstract
We have combined the circular chromosome conformation capture protocol with high-throughput, genome-wide sequence analysis to characterize the cis-acting regulatory network at a single locus. In contrast to methods which identify large interacting regions (10-1000 kb), the 4C approach provides a comprehensive, high-resolution analysis of a specific locus with the aim of defining, in detail, the cis-regulatory elements controlling a single gene or gene cluster. Using the human α-globin locus as a model, we detected all known local and long-range interactions with this gene cluster. In addition, we identified two interactions with genes located 300 kb (NME4) and 625 kb (FAM173a) from the α-globin cluster.
摘要
我们将环形染色体构象捕获技术与高通量、全基因组序列分析相结合，以在单个基因座上对顺式作用调控网络进行特征分析。与识别大相互作用区域（10-1000kb）的方法不同，4C 方法可对特定基因座进行全面、高分辨率的分析，旨在详细定义控制单个基因或基因簇的顺式调控元件。我们使用人类α-珠蛋白基因座作为模型，检测了该基因座与所有已知的局部和长距离相互作用。此外，我们还鉴定了与位于α-珠蛋白基因座 300kb（NME4）和 625kb（FAM173a）处的基因的两个相互作用。
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