Burdeska A, Ott M, Bannwarth W, Then R L
Pharmaceutical Research Department, F. Hoffmann-La Roche Ltd., Basel, Switzerland.
FEBS Lett. 1990 Jun 18;266(1-2):159-62. doi: 10.1016/0014-5793(90)81529-w.
The nucleotide sequence of a 1.25 kb BglII/EcoRI fragment from the 34 kb trimethoprim (Tp)-resistant plasmid pABU1 of Staphylococcus aureus 157/4696, isolated in Zürich, was determined. It contained the entire Tp-resistant dihydrofolate reductase gene, 197 bp of the thymidylate synthetase, 395 bp of a truncated gene and 111 bp of IS257R1. With the exception of one single base pair at position of 862 the sequence of the whole fragment was identical to nucleotides 1633 to 2885 of the Tp-resistant transposon Tn4003 in plasmid pSK1 from an Australian S. aureus isolate. This suggests the worldwide dissemination of Tn4003 in multiresistant Staphylococci.
对从苏黎世分离出的金黄色葡萄球菌157/4696的34 kb甲氧苄啶（Tp）抗性质粒pABU1中一个1.25 kb的BglII/EcoRI片段的核苷酸序列进行了测定。它包含整个Tp抗性二氢叶酸还原酶基因、197 bp的胸苷酸合成酶、395 bp的一个截短基因和111 bp的IS257R1。除了第862位的一个单碱基对外，整个片段的序列与来自澳大利亚金黄色葡萄球菌分离株的质粒pSK1中Tp抗性转座子Tn4003的核苷酸1633至2885相同。这表明Tn4003在多重耐药葡萄球菌中在全球范围内传播。
