[电针“天枢”（ST 25）、“曲池”（LI 11）和“上巨虚”（ST 37）及其配对穴位对大鼠胃动力的影响]

[Influence of electroacupuncture stimulation of "tianshu" (ST 25), "quchi" (LI 11) and "shangjuxu" (ST 37) and their pairs on gastric motility in the rat].

作者信息

Yu Zhi, Xia You-Bing, Lu Ming-Xiang, Lin Jing, Yu Wen-Jing, Xu Bin

机构信息

Key Laboratory of Integrated Acupuncture and Drugs Constructed by Chinese Ministry of Education and Jiangsu Province, Nanjing University of Chinese Medicine, Nanjing 210029, China.

出版信息

Zhen Ci Yan Jiu. 2013 Feb;38(1):40-7.

PMID:23650799

Abstract

OBJECTIVE

To observe the effect of electroacupuncture (EA) stimulation of different acupoints or acupoint pairs on gastric motility so as to explore their modulation regularities under different conditions.

METHODS

SD rats were randomly divided into normal control, starvation (food-deprivation for 24 h), atropine (antagonist for M-receptor), acetylcholine (Ach, agonist for M-receptor), propranolol (antagonist for beta-receptor) and clenbuterol (agonist for beta 2-receptor) and paired-acupoint groups (30 rats/group). The intragastric pressure was measured via a pressure transducer connected to a balloon inserted in the stomach cavity. EA (2 Hz /15 Hz, 2 mA) was applied to the left "Tianshu" (ST 25),"Quchi" (LI 11) and "Shangjuxu" (ST 37) which were formed in pairs: ST 25-LI 11, ST 25-ST 37 and LI 11-ST 37 for 2 min following intravenous injection of atropine (0.1%, 0.8 mL/kg, 40 microL x min(-1) x kg(-1)), 0.1% acetylcholine (20 microL x min(-1) x kg(-1)), 0. 2% clenbuterol (80 microL x min(-1) x kg(-1)) and 0.4% propranolol (1 mL/kg,40 microL x min(-1) x kg(-1)) under food-deprivation conditions.

RESULTS

After intravenous injection of atropine and clenbuterol, the intragastric pressure were decreased significantly (P < 0.05), while after administration of Ach and propranolol, the intragastric pressure increased markedly (P < 0.05). Under normal and starvation conditions, and after intravenous administration of M-receptor antagonist (atropine) and agonist (Ach), beta-receptor antagonist (propranolol) and agonist (clenbuterol), EA stimulation of ST 25 produced an apparently inhibitory effects on gastric motility (80.00%, 86.67%, 76.67%, 86.67%, 73.33% and 86.67%, respectively) and intragatric pressure (P < 0.05) with the tendency being starvation > normal, acetylcholine > atropine and clenbuterol > propranolol. Whereas EA stimulation of LI 11 and ST 37 mainly produced an excitatory effect on gastric motility (60.00%, 56.67%, 93.33%, 40.00%, 53.33% and 50.00%, respectively for LI 11; 66.67%, 60.00%, 80.00%, 53.33%, 46.67% and 73.33%, respectively for ST 37). Following EA stimulation of the paired-acupoint groups, ST 25-ST 37 induced a predominately inhibitory effect on gastric motility (50.00%) and intragastric pressure, while LI 11-ST 37 stimulation had a principally excitatory effect on gastric motility (53.33%), and ST 25-LI 11 showed no apparent effect (50.00%).

CONCLUSION

EA stimulation of ST 25 area at the abdomen produces a predominant inhibitory effect on gastric motility, while EA of LI 11 and ST 37 on the upper and lower limbs induces an excitatory effect on gastric movement, when applied in pairs, EA of ST 25-ST 37 suppresses the gastric activity, and LI 11-ST 37 promotes the gastric activity, suggesting a specificity of the effect of different acupoint stimulation.

摘要

目的

观察电针刺激不同穴位或穴位组合对胃动力的影响，以探讨其在不同条件下的调节规律。

方法

将SD大鼠随机分为正常对照组、饥饿组（禁食24小时）、阿托品组（M受体拮抗剂）、乙酰胆碱组（M受体激动剂）、普萘洛尔组（β受体拮抗剂）、克伦特罗组（β2受体激动剂）和穴位配对组（每组30只大鼠）。通过连接插入胃腔的气囊的压力传感器测量胃内压。在饥饿条件下，静脉注射阿托品（0.1%，0.8 mL/kg，40 μL·min⁻¹·kg⁻¹）、0.1%乙酰胆碱（20 μL·min⁻¹·kg⁻¹）、0.2%克伦特罗（80 μL·min⁻¹·kg⁻¹）和0.4%普萘洛尔（1 mL/kg，40 μL·min⁻¹·kg⁻¹）后，对左侧“天枢”（ST25）、“曲池”（LI11）和“上巨虚”（ST37）组成的穴位组合ST25-LI11、ST25-ST37和LI11-ST37进行电针刺激（2 Hz/15 Hz，2 mA），持续2分钟。

结果

静脉注射阿托品和克伦特罗后，胃内压显著降低（P<0.05），而给予乙酰胆碱和普萘洛尔后，胃内压明显升高（P<0.05）。在正常和饥饿条件下，以及静脉注射M受体拮抗剂（阿托品）和激动剂（乙酰胆碱）、β受体拮抗剂（普萘洛尔）和激动剂（克伦特罗）后，电针刺激ST25对胃动力产生明显抑制作用（分别为80.00%、86.67%、76.67%、86.67%、73.33%和86.67%）和胃内压（P<0.05），趋势为饥饿>正常，乙酰胆碱>阿托品，克伦特罗>普萘洛尔。而电针刺激LI11和ST37主要对胃动力产生兴奋作用（LI11分别为60.00%、56.67%、93.33%、40.00%、53.33%和50.00%；ST37分别为66.67%、60.00%、80.00%、53.33%、46.67%和73.33%）。电针刺激穴位配对组后，ST25-ST37对胃动力（50.00%）和胃内压主要产生抑制作用，而LI11-ST37刺激对胃动力主要产生兴奋作用（53.33%），ST25-LI11无明显作用（50.00%）。