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电针足三里（ST36）对盲肠结扎穿刺所致脓毒症的影响及其与脾脏的相关性

Effect of Electroacupuncture at Zusanli (ST36) on Sepsis Induced by Cecal Ligation Puncture and Its Relevance to Spleen.

作者信息

Xie Dong-Ping, Zhou Geng-Biao, Chen Rui-Lan, Qin Xiao-Lian, Du Jiong-Dong, Zhang Yan, Weng Yan-Na, Mai Shu-Tao, Lai Fang, Han Yun

机构信息

The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, Guangdong, China.

Guangdong Provincial Hospital of Chinese Medicine, Guangzhou, Guangdong, China.

出版信息

Evid Based Complement Alternat Med. 2020 Oct 7;2020:1914031. doi: 10.1155/2020/1914031. eCollection 2020.

DOI:10.1155/2020/1914031
PMID:33082818
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7563055/
Abstract

BACKGROUND

Acupuncture at Zusanli (ST36), Quchi (LI11), and Tianshu (ST25) is commonly used in septic patients by traditional Chinese physicians. The protective effect of acupuncture at ST36 on the intestinal barrier is associated with Cholinergic Anti-Inflammatory Pathway (CAIP). However, its detailed mechanism and whether acupuncture at LI11 and ST25 have similar effects to ST36 remain unclear.

AIM

To explore the effects of electroacupuncture (EA) at ST36, LI11, and ST25 on septic rats and investigate the role of the spleen in the treatment of EA at ST36.

METHODS

A septic rat model caused by cecal ligation and puncture (CLP) and a postsplenectomy (SPX) CLP rat model were established. Rats were divided into nine groups depending on different treatments. Serum levels of TNF-, IL-10, D-lactic acidosis (D-LA), double amine oxidase (DAO), and T-lymphocyte subgroup level in intestinal lymph nodes were compared.

RESULTS

EA could not improve the 2-day survival of CLP rats. For CLP rats, EA at ST36 and LI11 significantly decreased the levels of TNF-, IL-10, DAO, and D-LA in serum and normalized intestinal T-cell immunity. For SPX CLP rats, EA at ST36 failed to reduce serum concentrations of TNF-, IL-10, and D-LA but increased the values of CD3CD4/CD3CD8 cells and Treg/Th17 cells.

CONCLUSIONS

EA at ST36 and LI11, respectively, could alleviate inflammation reaction, protect the intestinal barrier, and maintain intestinal T-cell function in septic rats. Spleen participated in the protective effect of EA at ST36 in sepsis.

摘要

背景

足三里（ST36）、曲池（LI11）和天枢（ST25）穴位针刺是中医治疗脓毒症患者常用的方法。ST36穴位针刺对肠道屏障的保护作用与胆碱能抗炎通路（CAIP）有关。然而，其具体机制以及LI11和ST25穴位针刺是否具有与ST36类似的作用尚不清楚。

目的

探讨电针刺激ST36、LI11和ST25穴位对脓毒症大鼠的影响，并研究脾脏在ST36穴位电针治疗中的作用。

方法

建立盲肠结扎穿孔（CLP）所致脓毒症大鼠模型和脾切除（SPX）后CLP大鼠模型。根据不同治疗方法将大鼠分为9组。比较血清中肿瘤坏死因子-α、白细胞介素-10、D-乳酸（D-LA）、双胺氧化酶（DAO）水平以及肠淋巴结中T淋巴细胞亚群水平。

结果

电针不能提高CLP大鼠的2天生存率。对于CLP大鼠，ST36和LI11穴位电针显著降低血清中肿瘤坏死因子-α、白细胞介素-10、DAO和D-LA水平，并使肠道T细胞免疫恢复正常。对于SPX CLP大鼠，ST36穴位电针未能降低血清中肿瘤坏死因子-α、白细胞介素-10和D-LA浓度，但增加了CD3CD4/CD3CD8细胞和调节性T细胞/辅助性T细胞17细胞的值。

结论

ST36和LI11穴位电针分别可减轻脓毒症大鼠的炎症反应，保护肠道屏障并维持肠道T细胞功能。脾脏参与了ST36穴位电针对脓毒症的保护作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e932/7563055/6490d61380da/ECAM2020-1914031.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e932/7563055/d9956840a36c/ECAM2020-1914031.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e932/7563055/0796ed586f74/ECAM2020-1914031.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e932/7563055/16e56f5408be/ECAM2020-1914031.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e932/7563055/6490d61380da/ECAM2020-1914031.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e932/7563055/d9956840a36c/ECAM2020-1914031.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e932/7563055/0796ed586f74/ECAM2020-1914031.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e932/7563055/16e56f5408be/ECAM2020-1914031.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e932/7563055/6490d61380da/ECAM2020-1914031.004.jpg

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