Uyanoglu Mustafa, Yamac Mustafa, Canbek Mediha, Senturk Hakan, Kartkaya Kazım, Oglakci Aysegul, Turgak Ozge, Kanbak Gungor
Eskisehir Osmangazi University, Science and Arts Faculty, Department of Biology, Eskisehir, Turkey.
Ultrastruct Pathol. 2013 May;37(3):218-26. doi: 10.3109/01913123.2013.786297.
Abstract The authors investigate the curative effects of crude exopolysaccharides (EPS) produced by four Basidomycetes strains on the symptoms of alcoholic liver injury. EPSs were administered to experimental groups at levels of 100 mg per kg body weight per day for 7 days using an oral zonde needle after the onset of the disease. Serum levels of alanine transaminase (ALT), lactate dehydrogenase (LDH), DNA fragmentation, caspase-3 activities, and mitochondrial outer membrane integrity were determined following sacrifice of the rats. Light and transmission electron microscope (TEM) studies were performed on liver sections for histopathological and cytological evaluations. EPS that was obtained from Coprinus comatus OBCC 1014 decreased serum ALT level and increased outer membrane integrity, and allowed for the regaining of histologically and cytologically normal hepatocyte and tissue views. As a result, based on the obtained data, it can be argued that among all studied mushroom strains crude exopolysaccharides from Coprinus comatus OBCC 1014 strain have therapeutic potential for alcoholic liver injury according to control groups.
摘要 作者研究了四种担子菌菌株产生的粗胞外多糖（EPS）对酒精性肝损伤症状的治疗效果。在疾病发作后，使用口服灌胃针以每天每千克体重100毫克的剂量给实验组施用EPS，持续7天。在处死大鼠后，测定血清丙氨酸转氨酶（ALT）、乳酸脱氢酶（LDH）水平、DNA片段化、半胱天冬酶-3活性和线粒体外膜完整性。对肝脏切片进行光镜和透射电子显微镜（TEM）研究，以进行组织病理学和细胞学评估。从毛头鬼伞OBCC 1014获得的EPS降低了血清ALT水平，增加了外膜完整性，并使肝细胞和组织在组织学和细胞学上恢复到正常状态。因此，根据所获得的数据，可以认为，在所有研究的蘑菇菌株中，与对照组相比，来自毛头鬼伞OBCC 1014菌株的粗胞外多糖对酒精性肝损伤具有治疗潜力。
