Uyanoglu Mustafa, Canbek Mediha, van Griensven Leo J L D, Yamac Mustafa, Senturk Hakan, Kartkaya Kazım, Oglakcı Aysegul, Turgak Ozge, Kanbak Gungor
Department of Biology, Science and Arts Faculty, Eskisehir Osmangazi University , Eskisehir , Turkey .
Int J Food Sci Nutr. 2014 Jun;65(4):482-8. doi: 10.3109/09637486.2013.869796. Epub 2014 Jan 7.
In the present study, the curative effects of crude polysaccharides (PSs) from mushrooms on the symptoms of alcoholic liver injury were investigated. PSs from Agaricus bisporus, Agaricus brasiliensis, and Phellinus linteus fruiting bodies were administered by gavage at levels of 100 mg per kg body weight per day for 7 d after the onset of the disease. The caspase-3 activity, mitochondrial membrane potential, mitochondrial outer membrane integrity of the liver tissues of sacrificed rats, and the serum alanine aminotransferase (ALT) levels were determined. In addition, light and transmission electron microscope (TEM) studies were performed for histopathological and cytological evaluations on liver sections. PSs from A. brasiliensis decreased ALT level and mitochondrial membrane potential and increased the outer membrane integrity; microscopic examinations also revealed normal hepatocytes and tissue. On the basis of our data, it can be argued that crude PSs from Agaricus brasiliensis have therapeutic potential for alcoholic liver injury.
在本研究中，对蘑菇粗多糖（PSs）治疗酒精性肝损伤症状的疗效进行了研究。在疾病发作后，将双孢蘑菇、巴西蘑菇和桑黄子实体中的PSs以每天每千克体重100毫克的剂量灌胃给药，持续7天。测定了处死大鼠肝脏组织的半胱天冬酶-3活性、线粒体膜电位、线粒体外膜完整性以及血清丙氨酸氨基转移酶（ALT）水平。此外，还对肝脏切片进行了光镜和透射电子显微镜（TEM）研究，以进行组织病理学和细胞学评估。巴西蘑菇中的PSs降低了ALT水平和线粒体膜电位，并提高了外膜完整性；显微镜检查还显示肝细胞和组织正常。根据我们的数据，可以认为巴西蘑菇粗PSs对酒精性肝损伤具有治疗潜力。
