Ozalp Fatma Ozgul, Canbek Mediha, Yamac Mustafa, Kanbak Gungor, Van Griensven Leo J L D, Uyanoglu Mustafa, Senturk Hakan, Kartkaya Kazım, Oglakci Aysegul
Department of Biology, Faculty of Science and Arts, Eskisehir Osmangazi University , Eskisehir , Turkey .
Pharm Biol. 2014 Aug;52(8):994-1002. doi: 10.3109/13880209.2013.874536. Epub 2014 Mar 6.
Excess use of alcohol is known to be associated with liver diseases such as fatty liver, alcoholic hepatitis, and cirrhosis. Various practices may be applied to prevent or treat the damage caused by chronic alcoholism. Coprinus comatus (O.F. Müll.) Pers. (Agaricaceae) is a macrofungus that has been reported to aid the recovery of murine livers damaged by benzopyrene.
In this study, the possible therapeutic effects of three different doses (50, 100, and 150 mg/kg) of C. comatus polysaccharide (PS) extract were studied in rats subjected to an alcoholic diet. The histological and biochemical results were compared between the control and experimental groups.
Modified Lieber-Decarli's calorie-adjusted liquid alcohol diet was given orally for 60 d. In addition to histopathology, alanine transaminase (ALT), aspartate transaminase (AST), mitochondrial membrane integrity, total cytochrome-c oxidase activity (TotalStCox), total mitochondrial cytochrome-c oxidase activity (TotalMtStCox), and caspase-3 values were used as liver parameters, and liver sections from all experimental groups were examined by electron microscopy.
Using histopathological assessment, it was observed that there was a decline in liver hepatocyte vacuolization in the treatment group fed 50 mg PS/kg. The TotalStCox and TotalMtStCox values of this group differed from the EtOH control group (p < 0.05).
Daily administration of 50 mg/kg of C. comatus PS extract considerably reduced the negative effects of alcohol on liver structure and function.
已知过量饮酒与肝脏疾病如脂肪肝、酒精性肝炎和肝硬化有关。可以采用各种方法来预防或治疗慢性酒精中毒造成的损害。毛头鬼伞（Coprinus comatus (O.F. Müll.) Pers.，蘑菇科）是一种大型真菌，据报道它有助于恢复受苯并芘损伤的小鼠肝脏。
在本研究中，研究了三种不同剂量（50、100和150毫克/千克）的毛头鬼伞多糖（PS）提取物对接受酒精饮食的大鼠的可能治疗效果。比较了对照组和实验组的组织学和生化结果。
经口给予改良的利伯 - 德卡里热量调整液体酒精饮食60天。除了组织病理学外，还将丙氨酸转氨酶（ALT）、天冬氨酸转氨酶（AST）、线粒体膜完整性、总细胞色素c氧化酶活性（TotalStCox）、总线粒体细胞色素c氧化酶活性（TotalMtStCox）和半胱天冬酶 - 3值用作肝脏参数，并通过电子显微镜检查所有实验组的肝脏切片。
通过组织病理学评估观察到，喂食50毫克PS/千克的治疗组肝脏肝细胞空泡化有所减少。该组的TotalStCox和TotalMtStCox值与乙醇对照组不同（p < 0.05）。
每天给予50毫克/千克的毛头鬼伞PS提取物可显著降低酒精对肝脏结构和功能的负面影响。
