用于基于核酸内切酶的核酸检测的探针设计规则和有效酶。
Probe design rules and effective enzymes for endonuclease-based detection of nucleic acids.
机构信息
University of Maryland, College Park, MD 20742, USA.
出版信息
Bioorg Med Chem. 2013 Oct 15;21(20):6181-5. doi: 10.1016/j.bmc.2013.04.009. Epub 2013 Apr 15.
Junction probe (JP) platform is an isothermal endonuclease-based detection assay for both RNA and DNA. Herein, we screen 31 REAse and identify effective restriction endonucleases that can be used for JP detection. Secondly, we investigate how different probe architectures affect JP cleavage rates and conclude that although molecular beacon (MB) JP probes give less background noise than linear JP probes, the cleavage of MB JP probes are slower than linear JP probes.
连接探针（JP）平台是一种基于等温内切酶的 RNA 和 DNA 检测分析方法。在此，我们筛选了 31 种 REAse，并鉴定出可用于 JP 检测的有效限制内切酶。其次，我们研究了不同的探针结构如何影响 JP 的切割速率，并得出结论，尽管分子信标（MB）JP 探针比线性 JP 探针产生的背景噪声更小，但 MB JP 探针的切割速度比线性 JP 探针慢。
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