State Key Laboratory for Physical Chemistry of Solid Surfaces, The Key Laboratory for Chemical Biology of Fujian Province, and Department of Chemistry, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China.
Biosens Bioelectron. 2013 Oct 15;48:94-9. doi: 10.1016/j.bios.2013.03.082. Epub 2013 Apr 11.
Noninvasive monitoring of glucose in tears is highly desirable in tight glucose control. The polymerized crystalline colloidal array (PCCA) that can be incorporated into contact lens represents one of the most promising materials for noninvasive monitoring of glucose in tears. However, low sensitivity and slow time response of the PCCA reported in previous arts has limited its clinical utility. This paper presents a new PCCA, denoted as NIR-PCCA, comprising a CCA of glucose-responsive sub-micrometered poly(styrene-co-acrylamide-co-3-acrylamidophenylboronic acid) microgels embedded within a slightly positive charged hydrogel matrix of poly(acrylamide-co-2-(dimethylamino)ethyl acrylate). This newly designed NIR-PCCA can reflect near-infrared (NIR) light, whose intensity (at 1722 nm) would decrease evidently with increasing glucose concentration over the physiologically relevant range in tears. The lowest glucose concentration reliably detectable was as low as ca. 6.1 μg/dL. The characteristic response time τ(sensing) was 22.1±0.2s when adding glucose to 7.5 mg/dL, and the higher the glucose concentration is, the faster the time response. Such a rationally designed NIR-PCCA is well suited for ratiometric NIR sensing of tear glucose under physiological conditions, thereby likely to bring this promising glucose-sensing material to the forefront of analytical devices for diabetes.
在严格的血糖控制中，非常希望能够无创监测泪液中的葡萄糖。可掺入隐形眼镜的聚合结晶胶体阵列（PCCA）是无创监测泪液中葡萄糖的最有前途的材料之一。然而，以前的研究中报道的 PCCA 灵敏度低和响应时间慢限制了其临床应用。本文提出了一种新的 PCCA，记为 NIR-PCCA，它由包含在略微带正电荷的聚（丙烯酰胺-co-2-（二甲氨基）乙基丙烯酰胺）水凝胶基质中的葡萄糖响应亚微米级聚（苯乙烯-co-丙烯酰胺-co-3-丙烯酰胺基苯硼酸）微凝胶的 CCA 组成。这种新设计的 NIR-PCCA 可以反射近红外（NIR）光，其强度（在 1722nm 处）随着泪液中生理相关范围内葡萄糖浓度的增加而明显降低。可靠检测到的最低葡萄糖浓度低至约 6.1μg/dL。当向 7.5mg/dL 添加葡萄糖时，特征响应时间 τ（感测）为 22.1±0.2s，葡萄糖浓度越高，响应时间越快。这种合理设计的 NIR-PCCA 非常适合在生理条件下进行泪液葡萄糖的比率型 NIR 感测，从而可能使这种有前途的葡萄糖传感材料成为糖尿病分析设备的前沿。
