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研究基因表达：数据库搜索及启动子融合以探究细菌中的转录调控

Studying gene expression: database searches and promoter fusions to investigate transcriptional regulation in bacteria.

作者信息

Martinez-Vaz Betsy M, Makarevitch Irina, Stensland Shane

机构信息

Department of Biology, Hamline University, Saint Paul, MN 55104.

出版信息

J Microbiol Biol Educ. 2010 May 20;11(1):42-9. doi: 10.1128/jmbe.v11.i1.101. Print 2010.

DOI:10.1128/jmbe.v11.i1.101
PMID:23653697
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3577243/
Abstract

A laboratory project was designed to illustrate how to search biological databases and utilize the information provided by these resources to investigate transcriptional regulation in Escherichia coli. The students searched several databases (NCBI Genomes, RegulonDB and EcoCyc) to learn about gene function, regulation, and the organization of transcriptional units. A fluorometer and GFP promoter fusions were used to obtain fluorescence data and measure changes in transcriptional activity. The class designed and performed experiments to investigate the regulation of genes necessary for biosynthesis of amino acids and how expression is affected by environmental signals and transcriptional regulators. Assessment data showed that this activity enhanced students' knowledge of databases, reporter genes and transcriptional regulation.

摘要

一个实验室项目旨在说明如何搜索生物数据库，并利用这些资源提供的信息来研究大肠杆菌中的转录调控。学生们搜索了几个数据库（NCBI基因组数据库、调控基因数据库RegulonDB和大肠杆菌代谢数据库EcoCyc），以了解基因功能、调控以及转录单元的组织。使用荧光计和绿色荧光蛋白（GFP）启动子融合来获取荧光数据并测量转录活性的变化。该班级设计并进行了实验，以研究氨基酸生物合成所需基因的调控，以及表达如何受到环境信号和转录调节因子的影响。评估数据表明，这项活动增强了学生对数据库、报告基因和转录调控的了解。

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