Liu Wen, Zhai Chengkai, Zhang Xiaoqiang, Zhang Hong, Jiang Mingxia, Zhou Siyu, Liu Yang, Zhao Na, Zhao Junhong
Department of Nutrition and Food Hygiene, School of Public Health, Southeast University, Nanjing 210009, China.
Wei Sheng Yan Jiu. 2013 Mar;42(2):196-202.
To discuss the possible mechanism of the whole grain-soybean compound package on dyslipidaemia rats.
44 SD rats were randomly divided into four groups: the hyperlipidaemic group, the rice-flour group, the whole grain-soybean compound package group and the negative control group by lipid profiles, fed with corresponding feed for eight weeks. Serum total cholesterol (TC), triglyceride (TG), high density lipoprotein cholesterol (HDL-C) and low density lipoprotein cholesterol (LDL-C) were measured before and after the test. At the end of the experiment, blood samples were taken from the femoral artery, the rat organs were collected and weighted. Serum Visfatin levels and SREBP-2 mRNA and LDLR mRNA in the liver were measured.
Compared with the hyperlipidaemic group and rice-flour group, the body weight, serum TC, TG and LDL-C of whole grain-soybean compound package group were significantly decreased (P < 0.05), HDL-C was significantly increased (P < 0.05). Visfatin levels were significantly decreased (P < 0.05). Gene expressions of SREBP-2 and LDLR were significantly increased (P < 0.05). Gene expression of Visfatin in whole grain-soybean compound package group was significantly lower than that in hyperlipidaemia group and rice-flour group (P < 0.05).
Whole grain-soybean compound package can improve the serum lipid profiles and Visfatin of rats fed with a high fat diet. The possible mechanism is that the whole grain-soybean compound package can activate the expression of SREBP-2, LDLR and Visfatin. And then enhance the expressing activity of regulate the cholesterol metabolism by SREBP-2, LDLR and Visfatin. Ultimately, to reduce the level of rats' cholesterol, and then ameliorate the dyslipidaemia of rats.
探讨全谷物 - 大豆复合包装对高脂血症大鼠的可能作用机制。
将44只SD大鼠按血脂水平随机分为四组：高脂血症组、米粉组、全谷物 - 大豆复合包装组和阴性对照组，给予相应饲料喂养8周。检测试验前后血清总胆固醇（TC）、甘油三酯（TG）、高密度脂蛋白胆固醇（HDL - C）和低密度脂蛋白胆固醇（LDL - C）。实验结束时，从股动脉取血样，收集大鼠器官并称重。检测血清内脂素水平以及肝脏中SREBP - 2 mRNA和LDLR mRNA。
与高脂血症组和米粉组相比，全谷物 - 大豆复合包装组大鼠体重、血清TC、TG和LDL - C显著降低（P < 0.05），HDL - C显著升高（P < 0.05）。内脂素水平显著降低（P < 0.05）。SREBP - 2和LDLR基因表达显著增加（P < 0.05）。全谷物 - 大豆复合包装组内脂素基因表达显著低于高脂血症组和米粉组（P < 0.05）。
全谷物 - 大豆复合包装可改善高脂饮食喂养大鼠的血脂水平和内脂素。可能机制是全谷物 - 大豆复合包装可激活SREBP - 2、LDLR和内脂素的表达。进而增强SREBP - 2、LDLR和内脂素调节胆固醇代谢的表达活性。最终降低大鼠胆固醇水平，改善大鼠血脂异常。
