Dikshith T S, Raizada R B, Mulchandani N B
Industrial Toxicology Research Centre, Lucknow, India.
Indian J Exp Biol. 1990 Mar;28(3):208-12.
Administration of pure alkaloid of T. asthamatica, suspended in peanut oil and given in single doses (12-100 mg/kg) by gavage, to male rats caused inactivity, respiratory distress, salivation, nasal discharge and diarrhoea. The oral LD50 value of the alkaloid was 35.32 mg/kg. In short term toxicity study daily doses of the alkaloid (1.25, 2.5, 5 and 10 mg/kg) were given to male rats for 15 days. Smaller doses of the alkaloid (1.25 and 2.5 mg/kg/day) produced no signs of poisoning or death in animals; while 5 mg/kg/day produced signs of poisoning and death of two animals, 10 mg/kg/day caused death of all the animals within 7 days. Activities of glutamic oxaloacetic transaminase, glutamic pyruvic transaminase and alkaline phosphatase were significant and associated with morphological changes in liver. The alkaloid also caused marked changes in the morphology of seminiferous tubules and spermatogenic activity of experimental animals. Since the alkaloid is effective in microgram quantities, the non toxic effects observed after daily doses of 1.25 mg/kg in male rats assume great therapeutic significance.
将哮喘马兜铃纯生物碱悬浮于花生油中，以单剂量（12 - 100毫克/千克）经口灌胃给予雄性大鼠，可导致其活动减少、呼吸窘迫、流涎、鼻分泌物增多及腹泻。该生物碱的经口半数致死量（LD50）值为35.32毫克/千克。在短期毒性研究中，将该生物碱的日剂量（1.25、2.5、5和10毫克/千克）给予雄性大鼠，持续15天。较小剂量的生物碱（1.25和2.5毫克/千克/天）未在动物身上产生中毒迹象或死亡；而5毫克/千克/天导致动物出现中毒迹象并致使两只动物死亡，10毫克/千克/天在7天内导致所有动物死亡。谷氨酸草酰乙酸转氨酶、谷氨酸丙酮酸转氨酶和碱性磷酸酶的活性显著，并与肝脏的形态学变化相关。该生物碱还使实验动物的生精小管形态和生精活性发生显著变化。由于该生物碱在微克量时即有效，因此在雄性大鼠中每日给予1.25毫克/千克后观察到的无毒作用具有重大治疗意义。
