Department of Pharmacology, Institute of Basic Medical Sciences, Khyber Medical University, Peshawar, KPK, Pakistan.
BMC Complement Altern Med. 2013 Jun 17;13:135. doi: 10.1186/1472-6882-13-135.
We have previously reported that aerial parts of Tylophora hirsuta have antispasmodic profile. The current work is an attempt for isolation of pharmacologically active compound(s) that contribute for its antispasmodic activity.
Preliminary phytochemical screening for crude methanol extract of Tylophora hirsuta (Th.Cr) is performed. Brine shrimp cytotoxicity of crude methanol extract is performed. Column chromatography was used for isolation of compounds. Mass spectroscopy, H(1) NMR and C(13) NMR were used for structural determination of compounds. α-amyrin acetate was tried for possible spasmolytic activity in rabbit's jejunal preparations and KCl-induced contractions.
Th.Cr tested positive for saponins, alkaloids, flavonoids and terpenoids. Compound 1 was isolated as α-amyrin acetate. Compound 2 was heptaeicosanol. Crude methanol extract tested positive for brine shrimp cytotoxicity with LC(50) 492.33± 8.08 mg/ml. Compound 1 tested positive for antispasmodic activity on spontaneous rabbits' jejunum preparations with EC(50) (60 ± 2) × 10(-5)M. The compound also tested positive on KCl induced contractions with EC(50) (72 ± 3) × 10(-5)M.
The present work confirms that α-amyrin acetate is has antispasmodic profile and the relaxant effect may be attributed to α-amyrin acetate which is a major compound.
我们之前报道过，白星海芋的地上部分具有抗痉挛作用。目前的工作是试图分离出对其抗痉挛活性有贡献的药理活性化合物。
对白星海芋（Th.Cr）粗甲醇提取物进行初步的植物化学筛选。对白星海芋粗甲醇提取物的卤虫细胞毒性进行检测。采用柱层析法对化合物进行分离。利用质谱、H（1）NMR 和 C（13）NMR 对化合物进行结构鉴定。α-香树脂醇乙酸酯用于兔空肠标本的可能解痉活性和 KCl 诱导的收缩实验。
Th.Cr 对皂苷、生物碱、黄酮类化合物和萜类化合物呈阳性反应。化合物 1 被分离为α-香树脂醇乙酸酯。化合物 2 为正十七烷醇。粗甲醇提取物对卤虫细胞毒性呈阳性，LC（50）为 492.33±8.08mg/ml。化合物 1 对兔自发性空肠标本具有阳性的抗痉挛活性，EC（50）为（60±2）×10（-5）M。该化合物对 KCl 诱导的收缩也呈阳性，EC（50）为（72±3）×10（-5）M。
本工作证实了α-香树脂醇乙酸酯具有抗痉挛作用，其松弛作用可能归因于主要化合物α-香树脂醇乙酸酯。
