Allen A M, Yamada H, Mendelsohn F A
Department of Medicine, University of Melbourne, Austin Hospital, Heidelberg, Victoria, Australia.
Int J Cardiol. 1990 Jul;28(1):25-33. doi: 10.1016/0167-5273(90)90005-p.
The distribution of binding to angiotensin II receptors in the rat heart was determined by quantitative in vitro autoradiography employing 125I-[Sar1, Ile8] angiotensin II as the radioligand. Low density binding occurs in the myocardium of both the atriums and ventricles and in the media of the aorta, pulmonary arteries and superior caval vein. Dense punctate binding is found over parasympathetic nerve bundles and some cells of the intracardiac ganglions. Binding is very low in nerves which do not stain for acetylcholinesterase. A moderate to high density of binding sites occurs throughout the conduction system, including the sinus node (low), the atrioventricular node (high) and the atrioventricular bundle (moderate). An extremely high density of binding is observed over the remnant of the arterial duct. These findings demonstrate many sites at which angiotensin II could exert its cardiac actions, including anatomical evidence supporting an action on cardiac myocytes, terminals of the vagal nerves, parasympathetic ganglion cells, and the conduction system.
采用¹²⁵I-[Sar¹, Ile⁸]血管紧张素II作为放射性配体，通过定量体外放射自显影法测定了大鼠心脏中血管紧张素II受体的结合分布。低密度结合出现在心房和心室的心肌以及主动脉、肺动脉和上腔静脉的中膜。在副交感神经束和心内神经节的一些细胞上发现有密集的点状结合。在不被乙酰胆碱酯酶染色的神经中结合非常低。整个传导系统包括窦房结（低）、房室结（高）和房室束（中等）都出现中度到高密度的结合位点。在动脉导管残端观察到极高密度的结合。这些发现表明血管紧张素II可以发挥其心脏作用的许多部位，包括支持对心肌细胞、迷走神经末梢、副交感神经节细胞和传导系统作用的解剖学证据。
