In vitro autoradiographic localization of binding to angiotensin receptors in the rat heart.

The distribution of binding to angiotensin II receptors in the rat heart was determined by quantitative in vitro autoradiography employing 125I-[Sar1, Ile8] angiotensin II as the radioligand. Low density binding occurs in the myocardium of both the atriums and ventricles and in the media of the aorta, pulmonary arteries and superior caval vein. Dense punctate binding is found over parasympathetic nerve bundles and some cells of the intracardiac ganglions. Binding is very low in nerves which do not stain for acetylcholinesterase. A moderate to high density of binding sites occurs throughout the conduction system, including the sinus node (low), the atrioventricular node (high) and the atrioventricular bundle (moderate). An extremely high density of binding is observed over the remnant of the arterial duct. These findings demonstrate many sites at which angiotensin II could exert its cardiac actions, including anatomical evidence supporting an action on cardiac myocytes, terminals of the vagal nerves, parasympathetic ganglion cells, and the conduction system.