Laboratory of Microbial Ecology and Technology (LabMET), Ghent University, Gent, Belgium.
FEMS Microbiol Ecol. 2013 Sep;85(3):593-603. doi: 10.1111/1574-6941.12145. Epub 2013 Jun 10.
In drinking water (DW) and the distribution systems, bacterial growth and biofilm formation have to be controlled both for limiting taste or odour development and preventing clogging or biocorrosion problems. After a contamination with undesired bacteria, factors like nutrient availability and temperature will influence the survival of these invaders. Understanding the conditions enabling invaders to proliferate is essential for a holistic approach towards microbial risk assessment in DW. Pseudomonas putida was used as a model invader because this easy-growing bacterium can use a wide range of substrates. Invasion experiments in oligo- to eutrophic waters showed the requirement of both a carbon and phosphate source for survival of P. putida in DW. Addition of C, N and P enabled P. putida to grow in DW from 5.80 × 10(4) to 1.84 × 10(8) cells mL(-1) and survive for at least 12 days. However, in surface water with similar nutrient concentrations, P. putida did not survive, indicating the concomitant importance of the present indigenous microbial community of the specific water sample. Either extensive carbon or phosphate limitation can be used in water treatment design in order to obtain a DW which is not susceptible for unwanted bacterial growth.
在饮用水（DW）和分配系统中，必须控制细菌的生长和生物膜的形成，以限制味道或气味的发展，并防止堵塞或生物腐蚀问题。在受到不需要的细菌污染后，营养物质的可用性和温度等因素将影响这些入侵物的存活。了解使入侵物增殖的条件对于 DW 中微生物风险评估的整体方法至关重要。铜绿假单胞菌被用作入侵模型，因为这种易生长的细菌可以利用广泛的基质。在贫营养到富营养的水中的入侵实验表明，P. putida 在 DW 中生存需要碳源和磷酸盐源。添加 C、N 和 P 可使 P. putida 在 DW 中从 5.80×10(4)增长到 1.84×10(8)细胞/mL，并至少存活 12 天。然而，在具有相似营养浓度的地表水，P. putida 无法存活，这表明特定水样中当前土著微生物群落的同时重要性。在水处理设计中，可以使用广泛的碳或磷酸盐限制，以获得不易受不需要的细菌生长影响的 DW。
