Molecular Cell Physiology; Bielefeld University; Bielefeld, Germany.
Plant Signal Behav. 2013 Jul;8(7):e24638. doi: 10.4161/psb.24638. Epub 2013 May 10.
Alternative splicing (AS) gives rise to multiple mRNA isoforms from the same gene, providing possibilities to regulate gene expression beyond the level of transcription. In a recent paper in Nucleic Acids Research we used a high resolution RT-PCR based panel to study changes in AS patterns in plants with altered levels of an hnRNP-like RNA-binding protein in Arabidopsis thaliana. Furthermore, we detected significant changes in AS patterns between different Arabidopsis ecotypes. Here we investigated how small changes in ambient temperature affect AS. We found significant changes in AS for 12 of 28 investigated events (43%) upon transfer of Arabidopsis plants from 20°C to 16°C and for 6 of the 28 investigated events (21%) upon transfer from 20°C to 24°C.
选择性剪接 (AS) 可从同一基因产生多个 mRNA 异构体,从而提供了超越转录水平调节基因表达的可能性。在最近发表在《核酸研究》上的一篇论文中,我们使用了一种高分辨率 RT-PCR 为基础的方法,研究了拟南芥中 hnRNP 样 RNA 结合蛋白水平改变时 AS 模式的变化。此外,我们还检测到不同拟南芥生态型之间 AS 模式的显著变化。在这里,我们研究了环境温度的微小变化如何影响 AS。我们发现,在将拟南芥从 20°C 转移到 16°C 时,28 个研究事件中有 12 个(43%)发生了显著的 AS 变化,而在将拟南芥从 20°C 转移到 24°C 时,28 个研究事件中有 6 个(21%)发生了显著的 AS 变化。