Institute of Agrochemistry and Food Technology (IATA), Spanish National Research Council (CSIC), PO Box 73, 46100 Burjassot, Valencia, Spain.
Br J Nutr. 2013 Nov;110(10):1828-36. doi: 10.1017/S0007114513001098. Epub 2013 May 9.
Coeliac disease is an autoimmune disorder triggered by gluten intake, causing intestinal inflammation and mucosal damage commonly associated with the malabsorption of nutrients and ferropenic anaemia. The present study evaluates the effects of the oral administration of Bifidobacterium longum CECT 7347 on gliadin-mediated alterations in hepatic Fe deposition and Hb concentration, liver transferrin receptor (TfR)-2, IL-6, TNF-α and hepcidin (Hamp) expression (mRNA), and active hepcidin peptide production by liquid chromatography–MS/MS. Weanling rats, sensitised or not with interferon (IFN)-γ, were fed with gliadins and/or the bifidobacterial strain. Gliadin feeding increased hepatic Fe deposition; however, only gliadin-fed sensitised animals showed lower Hb concentrations than the controls. TfR2 expression decreased after gliadins were fed to both sensitised and non-sensitised animals,and restored by the administration of B. longum. These observations were accompanied by increases in IL-6 expression levels in all the treatment groups; however, TNF-α expression only increased significantly in animals fed gliadins alone or together with B. longum if they had previously been sensitised with IFN-γ. Liver expression levels of Hamp diminished in all cases to the lowest values in animals sensitised with IFN-γ after being fed with gliadins and/or bifidobacteria. In these animals, plasma Hamp active peptide concentrations significantly increased when compared with the controls. Significant correlations were calculated between Hamp expression and liver Fe contents (liver Fe = 1/0·0032 + 0·032 x Hamp(exp)), and Hb concentrations (Hb = 11·49 + 10·13 x (Hamp(exp))1/2). These data indicate that oral administration of B. longum ameliorates gliadin-mediated perturbations in liver Fe deposition and mobilisation.
乳糜泻是一种由麸质摄入引发的自身免疫性疾病，导致肠道炎症和黏膜损伤，通常与营养物质吸收不良和缺铁性贫血有关。本研究评估了口服长双歧杆菌 CECT7347 对麦胶介导的肝脏铁沉积和血红蛋白浓度、肝脏转铁蛋白受体 (TfR)-2、白细胞介素-6 (IL-6)、肿瘤坏死因子-α (TNF-α) 和铁调素 (Hamp) 表达 (mRNA) 以及通过液相色谱-质谱/质谱法产生的活性铁调素肽的影响。用干扰素 (IFN)-γ 致敏或未致敏的断奶大鼠用麦胶喂养和/或双歧杆菌菌株喂养。麦胶喂养增加了肝脏铁沉积；然而，只有麦胶喂养的致敏动物的血红蛋白浓度低于对照组。TfR2 表达在麦胶喂养的致敏和非致敏动物中均降低，而双歧杆菌的给药可恢复 TfR2 表达。这些观察结果伴随着所有治疗组中 IL-6 表达水平的增加；然而，仅在先前用 IFN-γ 致敏的动物中，仅在用麦胶单独或与双歧杆菌一起喂养时，TNF-α 表达才显著增加。在所有情况下，Hamp 的肝表达水平均降低，在用麦胶和/或双歧杆菌喂养的 IFN-γ 致敏动物中降至最低。在这些动物中，与对照组相比，血浆 Hamp 活性肽浓度显著增加。计算了 Hamp 表达与肝脏铁含量 (肝脏铁=1/0.0032+0.032x Hamp(exp)) 和血红蛋白浓度 (Hb=11.49+10.13x(Hamp(exp))1/2) 之间的显著相关性。这些数据表明，口服长双歧杆菌可改善麦胶介导的肝脏铁沉积和动员紊乱。
