Helmholtz Zentrum München-German Research Center for Environmental Health, Molecular Exposomics, Ingolstädter Landstr. 1, 85764 Neuherberg, Germany.
Anal Bioanal Chem. 2013 Jun;405(16):5571-82. doi: 10.1007/s00216-013-6952-8. Epub 2013 May 10.
Catecholamines play essential roles in several physiological processes in vertebrates as well as in invertebrates. While several studies have shown the presence of these substances in surface water invertebrates, their occurrence in groundwater fauna is unproven. In the present study, the presence of different catecholamines (i.e., noradrenaline, adrenaline, and dopamine) in individual specimens of groundwater amphipods of the genus Niphargus (mostly Niphargus inopinatus) was investigated via two independent analytical methods: HPLC/EcD and UPLC/TOF-MS. Mean values for catecholamine levels were 533 pg mg(-1) fresh weight for noradrenaline, 314 pg mg(-1) for adrenaline, and 16.4 ng mg(-1) for dopamine. The optimized protocol allowed the detection of CAs in single organisms of less than 1 mg fresh weight. Catecholamine concentration patterns in groundwater invertebrates are briefly discussed here with respect to their evolutionary adaptation to an environmentally stable, energy-poor habitat.
儿茶酚胺在脊椎动物和无脊椎动物的几个生理过程中发挥着重要作用。虽然有几项研究表明这些物质存在于地表水无脊椎动物中，但它们在地下水动物群中的存在尚未得到证实。在本研究中，通过两种独立的分析方法（HPLC/EcD 和 UPLC/TOF-MS）研究了地下水片脚类动物属（主要是 Niphargus inopinatus）个体标本中不同儿茶酚胺（即去甲肾上腺素、肾上腺素和多巴胺）的存在情况。去甲肾上腺素、肾上腺素和多巴胺的平均含量分别为 533 pg mg(-1)（鲜重）、314 pg mg(-1)和 16.4 ng mg(-1)。优化后的方案允许检测小于 1 毫克鲜重的单个生物体中的 CAs。本文简要讨论了地下水无脊椎动物中儿茶酚胺浓度模式，以了解它们对环境稳定、能量匮乏生境的进化适应。
