Department of Biomedical Engineering, Colorado State University, Fort Collins, CO 80523, USA.
Lab Chip. 2013 Jun 21;13(12):2397-404. doi: 10.1039/c3lc50072a. Epub 2013 May 8.
Paper-based analytical devices (PADs) represent a growing class of elegant, yet inexpensive chemical sensor technologies designed for point-of-use applications. Most PADs, however, still utilize some form of instrumentation such as a camera for quantitative detection. We describe here a simple technique to render PAD measurements more quantitative and straightforward using the distance of colour development as a detection motif. The so-called distance-based detection enables PAD chemistries that are more portable and less resource intensive compared to classical approaches that rely on the use of peripheral equipment for quantitative measurement. We demonstrate the utility and broad applicability of this technique with measurements of glucose, nickel, and glutathione using three different detection chemistries: enzymatic reactions, metal complexation, and nanoparticle aggregation, respectively. The results show excellent quantitative agreement with certified standards in complex sample matrices. This work provides the first demonstration of distance-based PAD detection with broad application as a class of new, inexpensive sensor technologies designed for point-of-use applications.
基于纸张的分析器件（PADs）代表了一类日益增长的优雅且廉价的化学传感器技术，专为现场应用而设计。然而，大多数 PAD 仍然使用某种形式的仪器，例如相机进行定量检测。我们在这里描述了一种简单的技术，通过将显色距离用作检测模式，使 PAD 测量更加定量和直接。与依赖外围设备进行定量测量的经典方法相比，这种所谓的基于距离的检测使 PAD 化学更加便携且资源消耗更少。我们使用三种不同的检测化学物质：酶反应、金属络合和纳米颗粒聚集，分别对葡萄糖、镍和谷胱甘肽的测量展示了该技术的实用性和广泛适用性。结果表明，在复杂的样品基质中，与认证标准具有极好的定量一致性。这项工作首次展示了基于距离的 PAD 检测，作为一类专为现场应用设计的新型廉价传感器技术具有广泛的应用前景。
