Department of Chemistry, Soochow University, Taipei 111, Taiwan.
Dalton Trans. 2017 May 30;46(21):6985-6993. doi: 10.1039/c7dt00875a.
In this work, we present a Ag@Au nanoprism-metal-organic framework-paper based glucose sensor for rapid, sensitive, single-use and quantitative glucose determination in human serum. To achieve painless measurement of glucose with a non-invasive detection methodology, this biosensor was further tested in human urine. In this approach, a new hybrid-Ag@Au nanoprism loaded in close proximity to micrometer sized coordination polymers as phosphorescent luminophores significantly enhanced the emission intensity due to metal-enhanced phosphorescence and worked as reaction sites to support more dissolved oxygen. Reports of enhanced phosphorescence intensity are relatively rare, especially at room temperature. The true enhancement factor of Ag@Au-phosphorescent metal-organic frameworks on paper was deduced to be 110-fold, making it a better optical type glucose meter. The results demonstrate the validity of the intensity enhancement effect of the excitation of the overlap of the emission band of a luminophore with the surface plasmon resonance band of Ag@Au nanoprisms. Ag@Au nanoprisms were used not only to improve the detection limit of glucose sensing but also to extend the glucose sensing range by enhancing the oxygen oxidation efficiency. The oxidation of glucose as glucose oxidase is accompanied by oxygen consumption, which increases the intensity of the phosphorescence emission. The turn-on type paper-based biosensor exhibits a rapid response (0.5 s), a low detection limit (0.038 mM), and a wide linear range (30 mM to 0.05 mM), as well as good anti-interference, long-term longevity and reproducibility. Finally, the biosensor was successfully applied to the determination of glucose in human serum and urine.
在这项工作中，我们提出了一种基于 Ag@Au 纳米棱镜-金属有机骨架-纸的葡萄糖传感器，用于快速、灵敏、一次性和定量测定人血清中的葡萄糖。为了实现无痛的非侵入性检测方法测量葡萄糖，该生物传感器进一步在人尿中进行了测试。在这种方法中，一种新的混合 Ag@Au 纳米棱镜负载在接近微米大小的配位聚合物作为磷光体，由于金属增强磷光显著增强了发射强度，并作为反应位点支持更多的溶解氧。增强磷光强度的报道相对较少，特别是在室温下。Ag@Au-磷光金属有机骨架在纸上的真实增强因子推断为 110 倍，使其成为更好的光学型血糖仪。结果表明，激发重叠的激发增强了磷光体的发射带与 Ag@Au 纳米棱镜的表面等离激元共振带之间的强度增强效应的有效性。Ag@Au 纳米棱镜不仅用于提高葡萄糖传感的检测限，而且通过增强氧气氧化效率来扩展葡萄糖传感范围。作为葡萄糖氧化酶的葡萄糖氧化伴随着氧气消耗，这增加了磷光发射的强度。开型纸基生物传感器具有快速响应（0.5 s）、低检测限（0.038 mM）和宽线性范围（30 mM 至 0.05 mM），以及良好的抗干扰性、长期寿命和重现性。最后，该生物传感器成功应用于人血清和尿液中葡萄糖的测定。
