Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, China.
Department of Laboratory Medicine, Zhongnan Hospital of Wuhan University, Wuhan 430071, China.
Biosens Bioelectron. 2012 May 15;35(1):363-368. doi: 10.1016/j.bios.2012.03.018. Epub 2012 Mar 17.
In this work, we first employ a drying method combining with the bienzyme colorimetric detection of glucose and uric acid on microfluidic paper-based analysis devices (μPADs). The channels of 3D μPADs are also designed by us to get better results. The color results are recorded by both Gel Documentation systems and a common camera. By using Gel Documentation systems, the limits of detection (LOD) of glucose and uric acid are 3.81 × 10(-5)M and 4.31 × 10(-5)M, respectively one order of magnitude lower than that of the reported methods on μPADs. By using a common camera, the limits of detection (LOD) of glucose and uric acid are 2.13 × 10(-4)M and 2.87 × 10(-4)M, respectively. Furthermore, the effects of detection conditions have been investigated and discussed comprehensively. Human serum samples are detected with satisfactory results, which are comparable with the clinical testing results. A low-cost, simple and rapid colorimetric method for the simultaneous detection of glucose and uric acid on the μPADs has been developed with enhanced sensitivity.
在这项工作中，我们首先采用结合微流控纸基分析器件（μPADs）上的双酶比色法检测葡萄糖和尿酸的干燥方法。我们还设计了 3D μPADs 的通道，以获得更好的结果。颜色结果由凝胶文档系统和普通相机记录。使用凝胶文档系统，葡萄糖和尿酸的检测限（LOD）分别为 3.81×10(-5)M 和 4.31×10(-5)M，比报道的μPADs 上的方法低一个数量级。使用普通相机，葡萄糖和尿酸的检测限（LOD）分别为 2.13×10(-4)M 和 2.87×10(-4)M。此外，还全面研究和讨论了检测条件的影响。用该方法检测人血清样品，结果令人满意，与临床检测结果相当。该方法具有成本低、操作简单、快速的特点，可增强灵敏度，用于μPADs 上葡萄糖和尿酸的同时比色检测。
