Department of Anatomy, School of Medicine, Kangwon National University, Chuncheon, 200-701, Korea.
J Assist Reprod Genet. 2013 Jun;30(6):835-41. doi: 10.1007/s10815-013-0007-0. Epub 2013 May 9.
Micro-vibration culture system was examined to determine the effects on mouse and human embryo development and possible improvement of clinical outcomes in poor responders.
The embryonic development rates and cell numbers of blastocysts were compared between a static culture group (n = 178) and a micro-vibration culture group (n = 181) in mice. The embryonic development rates and clinical results were compared between a static culture group (n = 159 cycles) and a micro-vibration culture group (n = 166 cycles) in poor responders. A micro-vibrator was set at a frequency of 42 Hz, 5 s/60 min duration for mouse and human embryo development.
The embryonic development rate was significantly improved in the micro-vibration culture group in mice (p < 0.05). The cell numbers of mouse blastocysts were significantly higher in the micro-vibration group than in the static culture group (p < 0.05). In the poor responders, the rate of high grade embryos was not significantly improved in the micro-vibration culture group on day 3. However, the optimal embryonic development rate on day 5 was improved in the micro-vibration group, and the total pregnancy rate and implantation rate were significantly higher in the micro-vibration group than in the static culture group (p < 0.05).
Micro-vibration culture methods have a beneficial effect on embryonic development in mouse embryos. In poor responders, the embryo development rate was improved to a limited extent under the micro-vibration culture conditions, but the clinical results were significantly improved.
研究微振动培养系统对小鼠和人胚胎发育的影响，以及对反应不良者临床结局的可能改善。
比较了小鼠中静态培养组（n=178）和微振动培养组（n=181）的胚胎发育率和囊胚细胞数。比较了反应不良者中静态培养组（n=159 个周期）和微振动培养组（n=166 个周期）的胚胎发育率和临床结果。为了促进小鼠和人类胚胎的发育，将微振动器设置为 42 Hz，60 分钟内持续 5 秒。
微振动培养组小鼠的胚胎发育率显著提高（p<0.05）。微振动组的囊胚细胞数明显高于静态培养组（p<0.05）。在反应不良者中，第 3 天微振动培养组优质胚胎率无显著提高。然而，第 5 天的最佳胚胎发育率在微振动组中得到了改善，并且微振动组的总妊娠率和着床率明显高于静态培养组（p<0.05）。
微振动培养方法对小鼠胚胎的胚胎发育有有益的影响。在反应不良者中，在微振动培养条件下，胚胎发育率得到了一定程度的提高，但临床结果有显著改善。
