YashRoy R C
Biology Department, Carleton University and N.R.C., Ottawa, Canada.
J Biochem Biophys Methods. 1990 Apr-May;20(4):353-6. doi: 10.1016/0165-022x(90)90097-v.
A new and simple approach for the determination of the temperature of gel-to-liquid crystalline phase transitions (Tc) of biological (chloroplast) membrane lipids from 13C-NMR resonance intensities is proposed. The variation of intensity of a temperature-sensitive NMR resonance is monitored by recording the spectra of the sample at a range of temperatures. From such a series of spectra recorded at different temperatures, a temperature-insensitive resonance is located. Then the ratio of the intensity of the temperature-sensitive to the intensity of the temperature-insensitive resonance is calculated from each spectrum to even out the procedural error, if any. The values of this ratio at different temperatures, when plotted against sample temperature, shows a break at Tc as confirmed by spin label ESR studies.
提出了一种新的简单方法，可根据13C核磁共振共振强度来测定生物（叶绿体）膜脂的凝胶-液晶相变温度（Tc）。通过在一系列温度下记录样品的光谱，监测对温度敏感的核磁共振共振强度的变化。从在不同温度下记录的这一系列光谱中，找到一个对温度不敏感的共振峰。然后从每个光谱中计算出对温度敏感的共振峰强度与对温度不敏感的共振峰强度之比，以消除可能存在的操作误差。将不同温度下该比值的值与样品温度作图时，如自旋标记电子顺磁共振研究证实的那样，在Tc处会出现一个拐点。
