Chemometrics, Qualimetric and Nanosensors Grup, Department of Analytical and Organic Chemistry, Rovia i Virgili University, Marcel·lí Domingo s/n, 43007 Tarragona, Spain.
Spectrochim Acta A Mol Biomol Spectrosc. 2013 Jul;111:237-41. doi: 10.1016/j.saa.2013.04.031. Epub 2013 Apr 11.
A substrate for Surface-Enhanced Raman Scattering spectroscopy (SERS), electropolished Al, is proposed as a tool for a rapid and low cost determination of Sudan I. This dye has been used as an additive in some foodstuffs but it is now banned because of the health risk associated with its carcinogenic and mutagenic properties. Despite the presence of fluorescence, Raman spectra of Sudan I can be obtained using excitation lasers at 633 and 785 nm. To get rid of the spectral noise and fluorescence background, Savitzky-Golay smoothing and polynomial corrections were applied, respectively. The Raman signal was proved to be enhanced. A linear dependence was found between the logarithmic intensity at 1598 cm(-1) peak versus the logarithmic concentration. The figures of merit were studied obtaining high sensitivity and low detection limits (10(-7) M). A multivariate exploratory analysis (PCA) was used to study the ability of SERS to distinguish Sudan I from other similar compounds. Therefore, results show that SERS is a potential tool to determine Sudan I quickly and effectively.
一种用于表面增强拉曼散射光谱（SERS）的基底——电化学抛光 Al 被提议作为一种快速、低成本测定苏丹红 I 的工具。该染料曾被用作某些食品的添加剂，但因其致癌和致突变特性带来的健康风险，现已被禁用。尽管苏丹红 I 存在荧光，但仍可使用 633nm 和 785nm 的激发激光获得其拉曼光谱。为了消除光谱噪声和荧光背景，分别应用了 Savitzky-Golay 平滑和多项式校正。结果证明拉曼信号得到了增强。在 1598cm(-1) 峰的对数强度与对数浓度之间发现了线性关系。研究了评价指标，获得了高灵敏度和低检测限（10(-7) M）。多元探索性分析（PCA）用于研究 SERS 区分苏丹红 I 和其他类似化合物的能力。因此，结果表明 SERS 是一种快速、有效地测定苏丹红 I 的潜在工具。
