Lohumi Santosh, Joshi Ritu, Kandpal Lalit Mohan, Lee Hoonsoo, Kim Moon S, Cho Hyunjeong, Mo Changyeun, Seo Young-Wook, Rahman Anisur, Cho Byoung-Kwan
a Department of Biosystems Machinery Engineering, College of Agricultural and Life Science , Chungnam National University , Yuseoung-gu , Korea.
b Environmental Microbial and Food Safety Laboratory , USDA-ARS , Beltsville , MD , USA.
Food Addit Contam Part A Chem Anal Control Expo Risk Assess. 2017 May;34(5):678-686. doi: 10.1080/19440049.2017.1290828. Epub 2017 Feb 17.
As adulteration of foodstuffs with Sudan dye, especially paprika- and chilli-containing products, has been reported with some frequency, this issue has become one focal point for addressing food safety. FTIR spectroscopy has been used extensively as an analytical method for quality control and safety determination for food products. Thus, the use of FTIR spectroscopy for rapid determination of Sudan dye in paprika powder was investigated in this study. A net analyte signal (NAS)-based methodology, named HLA/GO (hybrid linear analysis in the literature), was applied to FTIR spectral data to predict Sudan dye concentration. The calibration and validation sets were designed to evaluate the performance of the multivariate method. The obtained results had a high determination coefficient (R) of 0.98 and low root mean square error (RMSE) of 0.026% for the calibration set, and an R of 0.97 and RMSE of 0.05% for the validation set. The model was further validated using a second validation set and through the figures of merit, such as sensitivity, selectivity, and limits of detection and quantification. The proposed technique of FTIR combined with HLA/GO is rapid, simple and low cost, making this approach advantageous when compared with the main alternative methods based on liquid chromatography (LC) techniques.
由于经常报道食品中掺有苏丹红染料，尤其是含有辣椒粉和辣椒的产品，这一问题已成为解决食品安全问题的一个焦点。傅里叶变换红外光谱（FTIR）已被广泛用作食品质量控制和安全测定的分析方法。因此，本研究对利用FTIR光谱快速测定辣椒粉中苏丹红染料进行了研究。一种基于净分析物信号（NAS）的方法，即文献中所称的HLA/GO（混合线性分析），被应用于FTIR光谱数据以预测苏丹红染料浓度。校准集和验证集用于评估多元方法的性能。校准集得到的结果具有较高的决定系数（R），为0.98，较低的均方根误差（RMSE）为0.026%，验证集的R为0.97，RMSE为0.05%。该模型通过第二个验证集并利用诸如灵敏度、选择性以及检测限和定量限等品质因数进一步得到验证。所提出的FTIR与HLA/GO相结合的技术快速、简单且成本低，与基于液相色谱（LC）技术的主要替代方法相比，该方法具有优势。
