Riederer P, Bamberg E, Birkmayer W
Acta Vitaminol Enzymol. 1975;29(1-6):21-4.
The determination of free L-tryptophan depends on several analytical well checked points e.g. absorbing effects, pH of the serum, dependence of the throughput, concentration dependence of retention, volume ultrafiltrated, cut-off, temperature dependence of the binding rate, purification on ion-exchange column, interfering substances of the fluorometric method. Ultrafiltration was compared to an ultracentrifugation technique. In normal human serum the concentration of free L-tryptophan is 3,1 +/- 0,6 microgram/ml and of the bound plus free form 12,2 +/- 1,12 microgram/ml. Both values are dependent on the albumin concentration.
游离L-色氨酸的测定取决于几个经过充分验证的分析要点，例如吸收效应、血清的pH值、通量依赖性、保留的浓度依赖性、超滤体积、截留值、结合率的温度依赖性、离子交换柱纯化、荧光法的干扰物质。将超滤与超速离心技术进行了比较。在正常人血清中，游离L-色氨酸的浓度为3.1±0.6微克/毫升，结合型加游离型的浓度为12.2±1.12微克/毫升。这两个值均取决于白蛋白浓度。
